Characterization of the Fusarium sambucinum species complex and detection of multiple mycotoxins in Brazilian barley samples

Autor(es)

Iwase, Caio H. T. ^[1] ; Piacentini, Karim C. ^[1] ; Giomo, Patricia P. ^[1] ; Cumova, Martina ^[2] ; Wawroszova, Simona ^[2] ; Belakova, Sylvie ^[3] ; Minella, Euclydes ^[4] ; Rocha, Liliana O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Food Engn Fac, Dept Food Sci, UNICAMP, Campinas, SP - Brazil

^[2] Reg Dept Brno, Cent Inst Supervising & Testing Agr, Natl Reference Lab, Brno - Czech Republic

^[3] Malting Inst Brno, Res Inst Brewing & Malting, Brno - Czech Republic

^[4] Embrapa Trigo, Passo Fundo, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 136, OCT 2020.
• Citações Web of Science: 0

Resumo

This study investigated the fungal diversity in Brazilian barley samples, focusing on the Fusarium sambucinum species complex and the presence of multiple mycotoxins: aflatoxins B1, B2, G1, G2 beauvericin (BEA), enniatins (ENNs) A, A1, B, and B1, deoxynivalenol (DON), fumonisins (FB) B1 and B2, HT-2 and T-2 toxins, nivalenol (NIV) and ochratoxin A (OTA) from two different regions, Sao Paulo (SP) and Rio Grande do Sul (RS). The majority of the isolates belonged to the Fusarium sambucinum species complex (FSAMSC), with F. graminearum s.s. characterized as the major contaminant. F. meridionale and F. poae were the second most frequent fungi isolated from SP and RS, respectively. All of the F. graminearum s.s. isolates demonstrated 15-ADON genotype, whereas F. poae and F. meridionale were all NIV. The majority of the F. cortaderiae isolates were NIV, with only one 3-ADON genotype. Mycotoxin analysis revealed that none of the samples were contaminated by aflatoxins, OTA, FB2 and type A trichothecenes, however, all of the samples were contaminated with at least one Fusarium toxin. Contamination by DON, ZEA, ENNB and ENNB1 levels were significantly higher in RS. Co-contamination of BEA, DON, ENNs, NIV and ZEA in 18.5% and 24.2% of the analyzed samples was observed, from SP and RS respectively. More than 20% of the samples from RS presented DON and ZEA levels above the regulations established by Europe and Brazil. The results provide further information on the FSAMSC from South America and detected multiple Fusarium toxins in barley samples. This highlights the importance for further studies on the possible interactions of these mycotoxins in order to determine potential risks to animal health. (AU)

• Processo FAPESP: 17/04811-4 - Estrutura populacional do complexo de espécies Fusarium graminearum e perfil da contaminação por micotoxinas em cevada cultivada e selvagem do Brasil
• Beneficiário: Liliana de Oliveira Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores