Autor(es)

Valadao de Souza, Daniela Raguer ^[1] ; Pessoa, Rodrigo ^[1] ; Nascimento, Andrezza ^[1] ; Nukui, Youko ^[2] ; Pereira, Juliana ^[2] ; Casseb, Jorge ^[1] ; Penalva de Oliveira, Augusto Cesar ^[3] ; da Silva Duarte, Alberto Jose ^[1] ; Clissa, Patricia Bianca ^[4] ; Sanabani, Sabri Saeed ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Immunodeficiency, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Hematol, BR-05403000 Sao Paulo - Brazil

^[3] Emilio Ribas Inst Infectol, Dept Neurol, BR-01246900 Sao Paulo - Brazil

^[4] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Clin Hosp, Fac Med, Lab Med Invest Unit 03, 255 Eneas Carvalho Aguiar Ave, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oncology Letters; v. 20, n. 3, p. 2311-2321, SEP 2020.
• Citações Web of Science: 0

Resumo

In the present pilot study, massively parallel sequencing (MPS) technology was used to investigate cellular small RNA (sRNA) levels in the peripheral blood mononuclear cells (PBMCs) of human T-lymphotropic virus type I (HTLV-I) infected asymptomatic carriers with monoclonal (ASM) and polyclonal (ASP) T cell receptor (TCR) gamma gene. Blood samples from 15 HTLV-I asymptomatic carriers (seven ASM and eight ASP) were tested for the clonal TCR-gamma gene and submitted for sRNA library construction together with blood samples of five healthy controls (HCs) using Illumina sequencing platform. The sRNA-sequencing reads were aligned, annotated and profiled using various bioinformatics tools. Based on these results, possible markers were validated in the study samples by performing reverse transcription-quantitative (RT-q)PCR analysis. A total of 76 known sRNAs and 52 putative novel sRNAs were identified. Among them, 44 known and 34 potential novel sRNAs were differentially expressed in the ASM and ASP libraries compared with HCs. In addition, 10 known sRNAs were exclusively dysregulated in the ASM group and one (transfer RNA 65) was significantly upregulated in the ASP group.Homo sapiens(hsa) microRNA (miRNA/mir)-23a-3p, -28-5p, hsa-let-7e-5p and hsa-mir-28-3p and -361-5p were the most abundantly upregulated mature miRNAs and hsa-mir-363-3p, -532-5p, -106a-5p, -25-3p and -30e-5p were significantly downregulated miRNAs (P<0.05) with a >2-fold difference between the ASM and ASP groups compared with HCs. Based on these results, hsa-mir-23a-3p and -363-3p were selected for additional validation. However, the quantification of these two miRNAs using RT-qPCR did not provide any significant differences. While the present study failed to identify predictive sRNA markers to distinguish between ASM and ASP, the MPS results revealed differential sRNA expression profiles in the PBMCs of HTLV-1 asymptomatic carriers (ASM and ASP) compared with HCs. (AU)

• Processo FAPESP: 14/24596-2 - Sequenciamento em larga escala dos genomas do HIV na forma de RNA livre e DNA integrado: comparação entre os subtipos gerados, mutações relacionadas a resistência de drogas e o uso de co-receptores
• Beneficiário: Rodrigo Pessoa de Farias
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/12297-2 - Análise dos transcritos de miRNAs, REX e tax em células T no curso da infecção pelo HTLV-1, utilizando sequenciamento de nova geração
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular