| Full text | |
| Author(s): |
Valadao de Souza, Daniela Raguer
[1]
;
Pessoa, Rodrigo
[1]
;
Nascimento, Andrezza
[1]
;
Nukui, Youko
[2]
;
Pereira, Juliana
[2]
;
Casseb, Jorge
[1]
;
Penalva de Oliveira, Augusto Cesar
[3]
;
da Silva Duarte, Alberto Jose
[1]
;
Clissa, Patricia Bianca
[4]
;
Sanabani, Sabri Saeed
[5]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Immunodeficiency, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Dept Hematol, BR-05403000 Sao Paulo - Brazil
[3] Emilio Ribas Inst Infectol, Dept Neurol, BR-01246900 Sao Paulo - Brazil
[4] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil
[5] Univ Sao Paulo, Clin Hosp, Fac Med, Lab Med Invest Unit 03, 255 Eneas Carvalho Aguiar Ave, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Oncology Letters; v. 20, n. 3, p. 2311-2321, SEP 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
In the present pilot study, massively parallel sequencing (MPS) technology was used to investigate cellular small RNA (sRNA) levels in the peripheral blood mononuclear cells (PBMCs) of human T-lymphotropic virus type I (HTLV-I) infected asymptomatic carriers with monoclonal (ASM) and polyclonal (ASP) T cell receptor (TCR) gamma gene. Blood samples from 15 HTLV-I asymptomatic carriers (seven ASM and eight ASP) were tested for the clonal TCR-gamma gene and submitted for sRNA library construction together with blood samples of five healthy controls (HCs) using Illumina sequencing platform. The sRNA-sequencing reads were aligned, annotated and profiled using various bioinformatics tools. Based on these results, possible markers were validated in the study samples by performing reverse transcription-quantitative (RT-q)PCR analysis. A total of 76 known sRNAs and 52 putative novel sRNAs were identified. Among them, 44 known and 34 potential novel sRNAs were differentially expressed in the ASM and ASP libraries compared with HCs. In addition, 10 known sRNAs were exclusively dysregulated in the ASM group and one (transfer RNA 65) was significantly upregulated in the ASP group.Homo sapiens(hsa) microRNA (miRNA/mir)-23a-3p, -28-5p, hsa-let-7e-5p and hsa-mir-28-3p and -361-5p were the most abundantly upregulated mature miRNAs and hsa-mir-363-3p, -532-5p, -106a-5p, -25-3p and -30e-5p were significantly downregulated miRNAs (P<0.05) with a >2-fold difference between the ASM and ASP groups compared with HCs. Based on these results, hsa-mir-23a-3p and -363-3p were selected for additional validation. However, the quantification of these two miRNAs using RT-qPCR did not provide any significant differences. While the present study failed to identify predictive sRNA markers to distinguish between ASM and ASP, the MPS results revealed differential sRNA expression profiles in the PBMCs of HTLV-1 asymptomatic carriers (ASM and ASP) compared with HCs. (AU) | |
| FAPESP's process: | 14/24596-2 - HIV massively parallel sequencing data in plasma and peripheral blood mononuclear cells from untreated blood donors: comparison of the near full-length genome subtypes, drug resistance mutations and co-receptor usage |
| Grantee: | Rodrigo Pessoa de Farias |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 11/12297-2 - Profiling the human T-cells miRNA, REX and tax transcriptomes in the course of HTLV-1 infection using a deep sequencing approach |
| Grantee: | Sabri Saeed Mohammed Ahmed Al-Sanabani |
| Support Opportunities: | Regular Research Grants |