First isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil

Autor(es)

Alves, Vinicius Buiatte de Andrade ^[1] ; Carvalho, Eneas ^[2] ; Madureira, Paloma Alonso ^[1] ; Marino, Elizangela Domenis ^[1] ; Vaz, Andreia Cristina Nakashima ^[1] ; Vidal, Ana Maria Centola ^[1] ; Ruiz, Vera Letticie de Azevedo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Lab Doencas Infectoparasitarias Anim Domest, 225 Duque Caxias Av, BR-13635900 Pirassununga, SP - Brazil

^[2] Inst Butantan, Lab Bacteriol, 1500 Vital Brasil Av, BR-05503900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Microbiology; v. 20, n. 1 DEC 24 2020.
• Citações Web of Science: 0

Resumo

Background Infections caused by Shewanella spp. have been increasingly reported worldwide. The advances in genomic sciences have enabled better understanding about the taxonomy and epidemiology of this agent. However, the scarcity of DNA sequencing data is still an obstacle for understanding the genus and its association with infections in humans and animals. Results In this study, we report the first isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil using the boot sock method, as well as the resistance profile of this strain to antimicrobials. The isolate was first identified as Shewanella putrefaciens, but after whole-genome sequencing it showed greater similarity with Shewanella algae. The strain showed resistance to 46.7% of the antimicrobials tested, and 26 resistance genes were identified in the genome. Conclusions This report supports research made with Shewanella spp. and gives a step forward for understanding its taxonomy and epidemiology. It also highlights the risk of emerging pathogens with high resistance to antimicrobial formulas that are important to public health. (AU)

• Processo FAPESP: 19/19473-2 - Microbioma de fluido oral de suínos e suas relações com microbioma fecal
• Beneficiário: Paloma Alonso Madureira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/22184-7 - Microbioma de fluido oral de suínos e suas relações com microbioma fecal e presença de Salmonella enterica subsp. enterica.
• Beneficiário: Vera Letticie de Azevedo Ruiz
• Modalidade de apoio: Auxílio à Pesquisa - Regular