Genome sequence of Acremonium strictum AAJ6 strain isolated from the Cerrado biome in Brazil and CAZymes expression in thermotolerant industrial yeast for ethanol production

Autor(es):Mostrar menos -

Mendes Lopes, Alberto Moura ^[1] ; Felix de Melo, Allan Henrique ^[1] ; Procopio, Dielle Pierroti ^[1] ; Teixeira, Gleidson Silva ^[1] ; Carazzolle, Marcelo F. ^[2] ; de Carvalho, Lucas Miguel ^[2] ; Adelantado, Nuria ^{[3, 4]} ; Pereira, Goncalo A. G. ^[2] ; Ferrer, Pau ^{[3, 5]} ; Maugeri Filho, Francisco ^[1] ; Goldbeck, Rosana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Genom & Express Lab, Campinas, SP - Brazil

^[3] Univ Autonoma Barcelona UAB, Escola Engn, Dept Chem Biol & Environm Engn, Bellaterra, Catalonia - Spain

^[4] Lonza, Basel - Switzerland

^[5] Luxembourg Inst Sci & Technol LIST, Luxembourg - Luxembourg

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 98, p. 139-150, NOV 2020.
• Citações Web of Science: 0

Resumo

Increased demand for biofuels promotes the search for new biomass-degrading fungi. Acremonium strictum is an environmentally widespread filamentous fungi found on plant debris; that secretes lignocellulose-degrading enzymes. A recently isolated A. stricturn strain, AAJ6; native to the Brazilian Cerrado biome was evaluated for its capacity to degrade lignocellulosic substrates. In this study, whole-genome sequencing of AAJ6 was performed and 775 CAZy domains were identified which correlated to those of A. strictum strain DS1bioAY4a and other lignocellulolytic fungi; suggesting AAJ6 is a high CAZyme producer. We expressed the glycoside hydrolase families GH74 and GH3 from plasmid or genome-integrated to evaluate the ethanol production from cellulosic substrates in Brazilian industrial Saccharomyces cerevisiae strains (PE-2 and SA-1) evolved for thermotolerance (AMY12 and AMY35). Those expressing the genome-integrated enzymes showed the highest beta-glucosidase activity and growth in medium with cellobiose at 40 degrees C. The strain AGY005 (integrated cassettes) showed 19, 23 and 46% higher ethanol production in SHF, pSSF (partial hydrolysis SSF) and SSF processes, respectively, using Avicel, and similar to 50% more ethanol using pre-treated sugarcane bagasse, compared to the strain with a plasmid-based expression. These results indicate the improved performance of thermotolerant industrial strains with genome-integrated CAZymes in the SSF process for 2G ethanol. (AU)

• Processo FAPESP: 16/04602-3 - Desenvolvimento de linhagens de Saccharomyces cerevisiae através da expressão heteróloga de celulases e engenharia evolutiva visando a sacarificação e fermentação simultânea da biomassa lignocelulósica
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 15/02007-8 - Estudo da expressão heteróloga de celulases de Acremonium strictum em linhagens de Saccharomyces cerevisiae visando a produção de bioetanol de segunda geração
• Beneficiário: Dielle Pierotti Procópio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/02506-7 - Desenvolvimento de métodos high-throughput para o estudo de quantitative traits loci relacionados à robustez de leveduras industriais (Saccharomyces cerevisiae)
• Beneficiário: Gleidson Silva Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular