Creatine Supply Attenuates Ischemia-Reperfusion Injury in Lung Transplantation in Rats

Autor(es)

Almeida, Francine M. ^[1] ; Battochio, Angela S. ^[1] ; Napoli, Joao P. ^[2] ; Alves, Katiusa A. ^[2] ; Balbin, Grace S. ^[2] ; Oliveira-Junior, Manoel ^[3] ; Moriya, Henrique T. ^[4] ; Pego-Fernandes, Paulo M. ^[1] ; Vieira, Rodolfo P. ^{[3, 5, 6]} ; Pazetti, Rogerio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Inst Coracao, BR-05508060 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Pesquisa Cirurgia Torac LIM61, BR-05508060 Sao Paulo - Brazil

^[3] Brazilian Inst Teaching & Res Pulm & Exercise Imm, BR-04372020 Sao Jose Dos Campos - Brazil

^[4] Univ Sao Paulo, Biomed Engn Lab LEB, BR-05508060 Sao Paulo - Brazil

^[5] Univ Brasil, Postgrad Program Bioengn, BR-05403000 Sao Paulo - Brazil

^[6] Fed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, BR-04021001 Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 12, n. 9 SEP 2020.
• Citações Web of Science: 0

Resumo

Ischemia-reperfusion injury (IRI) is one of the factors limiting the success of lung transplantation (LTx). IRI increases death risk after transplantation through innate immune system activation and inflammation induction. Some studies have shown that creatine (Cr) protects tissues from ischemic damage by its antioxidant action. We evaluated the effects of Cr supplementation on IRI after unilateral LTx in rats. Sixty-four rats were divided into four groups: water + 90 min of ischemia; Cr + 90 min of ischemia; water + 180 min of ischemia; and Cr + 180 min of ischemia. Donor animals received oral Cr supplementation (0.5 g/kg/day) or vehicle (water) for five days prior to LTx. The left lung was exposed to cold ischemia for 90 or 180 min, followed by reperfusion for 2 h. We evaluated the ventilatory mechanics and inflammatory responses of the graft. Cr-treated animals showed a significant decrease in exhaled nitric oxide levels and inflammatory cells in blood, bronchoalveolar lavage fluid and lung tissue. Moreover, edema, cell proliferation and apoptosis in lung parenchyma were reduced in Cr groups. Finally, TLR-4, IL-6 and CINC-1 levels were lower in Cr-treated animals. We concluded that Cr caused a significant decrease in the majority of inflammation parameters evaluated and had a protective effect on the IRI after LTx in rats. (AU)

• Processo FAPESP: 12/15165-2 - Papel da sinalização purinérgica e da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelos experimentais de asma e em indivíduos asmáticos
• Beneficiário: Rodolfo de Paula Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/14848-1 - Efeitos da suplementação com creatina na lesão de isquemia e reperfusão após transplante pulmonar unilateral em ratos
• Beneficiário: Rogerio Pazetti
• Modalidade de apoio: Auxílio à Pesquisa - Regular