| Texto completo | |
| Autor(es): |
Tandonnet, Sophie
[1]
;
Cardoso, Gisele Antoniazzi
[1]
;
Mariano-Martins, Pedro
[1]
;
Monfardini, Raquel Dietsche
[1]
;
Cunha, Vanessa A. S.
[1]
;
de Carvalho, Renato Assis
[1, 2]
;
Torres, Tatiana Teixeira
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil
[2] Bayer Crop Sci, Rua Domingos Jorge 1100, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PARASITES & VECTORS; v. 13, n. 1 NOV 10 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
BackgroundThe emergence of insecticide resistance is a fast-paced example of the evolutionary process of natural selection. In this study, we investigated the molecular basis of resistance in the myiasis-causing fly Cochliomyia hominivorax (Diptera: Calliphoridae) to dimethyl-organophosphate (OP) insecticides.MethodsBy sequencing the RNA from surviving larvae treated with dimethyl-OP (resistant condition) and non-treated larvae (control condition), we identified genes displaying condition-specific polymorphisms, as well as those differentially expressed.ResultsBoth analyses revealed that resistant individuals have altered expression and allele-specific expression of genes involved in proteolysis (specifically serine-endopeptidase), olfactory perception and cuticle metabolism, among others. We also confirmed that resistant individuals carry almost invariably the Trp251Ser mutation in the esterase E3, known to confer OP and Pyrethroid resistance. Interestingly, genes involved in metabolic and detoxifying processes (notably cytochrome P450s) were found under-expressed in resistant individuals. An exception to this were esterases, which were found up-regulated.ConclusionsThese observations suggest that reduced penetration and aversion to dimethyl-OP contaminated food may be important complementary strategies of resistant individuals. The specific genes and processes found are an important starting point for future functional studies. Their role in insecticide resistance merits consideration to better the current pest management strategies. (AU) | |
| Processo FAPESP: | 14/13933-8 - Evolução do hábito alimentar na família Calliphoridae |
| Beneficiário: | Tatiana Teixeira Torres |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/06819-9 - Investigação das bases moleculares da resistência de Rhipicephalus microplus e Cochliomyia hominivorax a parasiticidas |
| Beneficiário: | Tatiana Teixeira Torres |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/00243-0 - Desenvolvimento de ferramentas computacionais para a comparação dos níveis de expressão entre as linhagens de pragas da pecuária |
| Beneficiário: | Sophie Hokulani Véronique Jessica Tandonnet |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |