Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly

Author(s)

Tandonnet, Sophie ^[1] ; Cardoso, Gisele Antoniazzi ^[1] ; Mariano-Martins, Pedro ^[1] ; Monfardini, Raquel Dietsche ^[1] ; Cunha, Vanessa A. S. ^[1] ; de Carvalho, Renato Assis ^{[1, 2]} ; Torres, Tatiana Teixeira ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil

^[2] Bayer Crop Sci, Rua Domingos Jorge 1100, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PARASITES & VECTORS; v. 13, n. 1 NOV 10 2020.
• Web of Science Citations: 0

Abstract

BackgroundThe emergence of insecticide resistance is a fast-paced example of the evolutionary process of natural selection. In this study, we investigated the molecular basis of resistance in the myiasis-causing fly Cochliomyia hominivorax (Diptera: Calliphoridae) to dimethyl-organophosphate (OP) insecticides.MethodsBy sequencing the RNA from surviving larvae treated with dimethyl-OP (resistant condition) and non-treated larvae (control condition), we identified genes displaying condition-specific polymorphisms, as well as those differentially expressed.ResultsBoth analyses revealed that resistant individuals have altered expression and allele-specific expression of genes involved in proteolysis (specifically serine-endopeptidase), olfactory perception and cuticle metabolism, among others. We also confirmed that resistant individuals carry almost invariably the Trp251Ser mutation in the esterase E3, known to confer OP and Pyrethroid resistance. Interestingly, genes involved in metabolic and detoxifying processes (notably cytochrome P450s) were found under-expressed in resistant individuals. An exception to this were esterases, which were found up-regulated.ConclusionsThese observations suggest that reduced penetration and aversion to dimethyl-OP contaminated food may be important complementary strategies of resistant individuals. The specific genes and processes found are an important starting point for future functional studies. Their role in insecticide resistance merits consideration to better the current pest management strategies. (AU)

• FAPESP's process: 14/13933-8 - Evolution of feeding preference in the Calliphoridae family
• Grantee: Tatiana Teixeira Torres
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/06819-9 - Investigation of the molecular basis of parasiticide resistance in Rhipicephalus microplus and Cochliomyia hominivorax
• Grantee: Tatiana Teixeira Torres
• Support Opportunities: Regular Research Grants