A semi-automated method for measuring xylem vessel length distribution

Autor(es):Mostrar menos -

Pereira, Luciano ^{[1, 2]} ; Miranda, Marcela T. ^{[1, 2]} ; Pires, Gabriel S. ^{[2, 3]} ; Pacheco, Vinicius S. ^[2] ; Guan, Xinyi ^[3] ; Kaack, Lucian ^[3] ; Karimi, Zohreh ^[4] ; Machado, Eduardo C. ^[1] ; Jansen, Steven ^[3] ; Tyree, Melvin T. ^[5] ; Ribeiro, Rafael V. ^[2]

Afiliação do(s) autor(es)

^[1] Agron Inst IAC, Ctr R&D Ecophysiol & Biophys, Lab Plant Physiol Coaracy M Franco, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Plant Biol, Inst Biol, Lab Crop Physiol, Campinas, SP - Brazil

^[3] Ulm Univ, Inst Systemat Bot & Ecol, Ulm - Germany

^[4] Golestan Univ, Dept Biol, Fac Sci, Gorgan, Golestan - Iran

^[5] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang - Peoples R China

• Tipo de documento: Artigo Científico
• Fonte: THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY; v. 32, n. 4, p. 331-340, DEC 2020.
• Citações Web of Science: 0

Resumo

Knowledge about the length of xylem vessels is essential to understand water transport in plants because these multicellular units show a 100-fold variation, from less than a centimeter to many meters. However, the available methods to estimate vessel length (VL) distribution are excessively time consuming and do not allow large and in-depth surveys. Here, we describe a semi-automated method to measure hydraulically-weighted VL (VLH) using an automated Pneumatron device. Gas conductivity of a xylem tissue with a certain length is estimated with the Pneumatron in a straightforward and precise way, theoretically similar to the air-injection method. Besides giving results similar to the silicone-injection method, the pneumatic-based method enables faster and easier measurements of more than 50 samples per day, which is a significant advantage. Herein, a detailed description of the methodology is presented as well as the software and an R-script for data analysis. The method described represents an important contribution to studies on plant hydraulic architecture and can improve our understanding about the role of VL(H)in plant performance under varying water availability. (AU)

• Processo FAPESP: 18/09834-5 - Vulnerabilidade ao embolismo em espécies cítricas: características hidráulicas, variação sazonal e relações entre copa e porta-enxerto
• Beneficiário: Marcela Trevenzoli Miranda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/15276-8 - Vulnerabilidade ao embolismo em espécies cítricas: características hidráulicas, variação sazonal e relações entre copa e porta-enxerto
• Beneficiário: Eduardo Caruso Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/14075-3 - Novos métodos para estudo das propriedades hidráulicas do xilema e embolismo in vivo em plantas
• Beneficiário: Luciano Pereira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado