OsteoBLAST: Computational Routine of Global Molecular Analysis Applied to Biomaterials Development

Autor(es)

Ferreira, Marcel Rodrigues ^[1] ; Milani, Renato ^[2] ; Rangel, Elidiane C. ^[3] ; Peppelenbosch, Maikel ^[4] ; Zambuzzi, Willian ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, Sao Paulo - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Bioquim & Biol Tecidual, Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Sao Paulo - Brazil

^[4] Erasmus MC, Univ Med Ctr Rotterdam, Dept Gastroenterol & Hepatol, Rotterdam - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 8, OCT 8 2020.
• Citações Web of Science: 0

Resumo

For bone purposes, surface modifications are a common trend in biomaterials research aiming to reduce the time necessary for osteointegration, culminating in faster recovery of patients. In this scenario, analysis of intracellular signaling pathways have emerged as an important and reliable strategy to predict biological responses from in vitro approaches. We have combined global analysis of intracellular protein phosphorylation, systems biology and bioinformatics into an early biomaterial analysis routine called OsteoBLAST. We employed the routine as follows: the PamChip tyrosine kinase assay was applied to mesenchymal stem cells grown on three distinct titanium surfaces: machined, dual acid-etched and nanoHA. Then, OsteoBLAST was able to identify the most reliable spots to further obtain the differential kinome profile and finally to allow a comparison among the different surfaces. Thereafter, NetworKIN, STRING, and Cytoscape were used to build and analyze a supramolecular protein-protein interaction network, and DAVID tools identified biological signatures in the differential kinome for each surface. (AU)

• Processo FAPESP: 18/05731-7 - PRP liofilizado associado à nano-hidroxiapatita na performance celular e regeneração óssea
• Beneficiário: Marcel Rodrigues Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/03639-8 - Mecanismos globais de transdução de sinais envolvidos com a resposta imediata a duas superfícies de ligas de titânio: construindo a base biodados OsteoBLAST
• Beneficiário: Marcel Rodrigues Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado