Overexpression of Citrus reticulata SAMT in Nicotiana tabacum increases MeSA volatilization and decreases Xylella fastidiosa symptoms

Autor(es)

Gomez, Laura M. ^{[1, 2]} ; Teixeira-Silva, Natalia S. ^[1] ; Caserta, Raquel ^[1] ; Takita, Marco A. ^[1] ; Marques, Marcia O. M. ^[3] ; de Souza, Alessandra A. ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Citricultura Sylvio Moreira IAC, Rodovia Anhanguera, Km 158, POB 04, BR-13490970 Cordeiropolis, SP - Brazil

^[2] Auburn Univ, Entomol & Plant Pathol Dept, Auburn, AL 36849 - USA

^[3] Dept Fitoquim IAC, Ave Doutor Theodureto Almeida Camargo 1500, BR-13012970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANTA; v. 252, n. 6 NOV 13 2020.
• Citações Web of Science: 0

Resumo

Main conclusion Nicotiana tabacum overexpressing CrSAMT from Citrus reticulata increased production of MeSA, which works as an airborne signal in neighboring wild-type plants, inducing PR1 and increasing resistance to the pathogen Xylella fastidiosa. Xylella fastidiosa is one of the major threats to plant health worldwide, affecting yield in many crops. Despite many efforts, the development of highly productive resistant varieties has been challenging. In studying host plant resistance, the S-adenosyl-l-methionine: salicylic acid carboxyl methyltransferase gene (SAMT) from Citrus reticulata, a X. fastidiosa resistant species, was upregulated in response to pathogen infection. SAMT is involved with the catalysis and production of methyl salicylate (MeSA), an airborne signal responsible for triggering systemic acquired resistance. Here we used tobacco as a model system and generated transgenic plants overexpressing C. reticulata SAMT (CrSAMT). We performed an in silico structural characterization of CrSAMT and investigated its biotechnological potential in modulating the immune system in transgenic plants. The increase of MeSA production in transgenic lines was confirmed by gas chromatography (GC-MS). The transgenic lines showed upregulation of PR1, and their incubation with neighboring wild-type plants activated PR1 expression, indicating that MeSA worked as an airborne signal. In addition, transgenic plants showed significantly fewer symptoms when challenged with X. fastidiosa. Altogether, these data suggest that CrSAMT plays a role in host defense response and can be used in biotechnology approaches to confer resistance against X. fastidiosa. (AU)

• Processo FAPESP: 19/01447-5 - Obtenção de porta enxerto transgênico: possibilidade de aumento de níveis de resistência da copa a fitopatógenos através da translocação de moléculas difusíveis
• Beneficiário: Natália de Sousa Teixeira e Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico
• Beneficiário: Alessandra Alves de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/16142-0 - Translocação de moléculas de quorum sensing de porta-enxertos transgênicos para copas de laranja doce: efeito quanto à resistência a fitopatógenos
• Beneficiário: Raquel Caserta Salviatto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/20422-0 - Avaliação funcional do gene SAMT (ácido salicílico carboxil metiltransferase) de Citrus reticulata na resistência a fitopatógenos de citros através da superexpressão em Nicotiana tabacum
• Beneficiário: Laura Melissa Gómez Krapp
• Modalidade de apoio: Bolsas no Brasil - Mestrado