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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Overexpression of Citrus reticulata SAMT in Nicotiana tabacum increases MeSA volatilization and decreases Xylella fastidiosa symptoms

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Author(s):
Gomez, Laura M. [1, 2] ; Teixeira-Silva, Natalia S. [1] ; Caserta, Raquel [1] ; Takita, Marco A. [1] ; Marques, Marcia O. M. [3] ; de Souza, Alessandra A. [1]
Total Authors: 6
Affiliation:
[1] Ctr Citricultura Sylvio Moreira IAC, Rodovia Anhanguera, Km 158, POB 04, BR-13490970 Cordeiropolis, SP - Brazil
[2] Auburn Univ, Entomol & Plant Pathol Dept, Auburn, AL 36849 - USA
[3] Dept Fitoquim IAC, Ave Doutor Theodureto Almeida Camargo 1500, BR-13012970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLANTA; v. 252, n. 6 NOV 13 2020.
Web of Science Citations: 0
Abstract

Main conclusion Nicotiana tabacum overexpressing CrSAMT from Citrus reticulata increased production of MeSA, which works as an airborne signal in neighboring wild-type plants, inducing PR1 and increasing resistance to the pathogen Xylella fastidiosa. Xylella fastidiosa is one of the major threats to plant health worldwide, affecting yield in many crops. Despite many efforts, the development of highly productive resistant varieties has been challenging. In studying host plant resistance, the S-adenosyl-l-methionine: salicylic acid carboxyl methyltransferase gene (SAMT) from Citrus reticulata, a X. fastidiosa resistant species, was upregulated in response to pathogen infection. SAMT is involved with the catalysis and production of methyl salicylate (MeSA), an airborne signal responsible for triggering systemic acquired resistance. Here we used tobacco as a model system and generated transgenic plants overexpressing C. reticulata SAMT (CrSAMT). We performed an in silico structural characterization of CrSAMT and investigated its biotechnological potential in modulating the immune system in transgenic plants. The increase of MeSA production in transgenic lines was confirmed by gas chromatography (GC-MS). The transgenic lines showed upregulation of PR1, and their incubation with neighboring wild-type plants activated PR1 expression, indicating that MeSA worked as an airborne signal. In addition, transgenic plants showed significantly fewer symptoms when challenged with X. fastidiosa. Altogether, these data suggest that CrSAMT plays a role in host defense response and can be used in biotechnology approaches to confer resistance against X. fastidiosa. (AU)

FAPESP's process: 19/01447-5 - Getting transgenic rootstock: possibility of increasing levels of scion resistance to phytopathogens through the translocation of diffusible molecules
Grantee:Natália de Sousa Teixeira e Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/10957-0 - Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control
Grantee:Alessandra Alves de Souza
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/16142-0 - Translocation of quorum sensing molecules from transgenic rootstocks to sweet orange canopies: effect on resistance against phytopathogens
Grantee:Raquel Caserta Salviatto
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/20422-0 - Functional analysis of citrus reticulata SAMT gene in the resistance of citrus phytopathogen through overexpression in Nicotiana tabacum
Grantee:Laura Melissa Gómez Krapp
Support Opportunities: Scholarships in Brazil - Master