Metamorphic acceleration following the exposure to lithium and selenium on American bullfrog tadpoles (Lithobates catesbeianus)

Autor(es)

Pinto Vidal, Felipe Augusto ^{[1, 2]} ; Abdalla, Fabio Camargo ^[3] ; Carvalho, Cleoni dos Santos ^[4] ; Moraes Utsunomiya, Heidi Samantha ^[4] ; Teixeira Oliveira, Leonardo Antonio ^[1] ; Salla, Raquel Fernanda ^[5] ; Jones-Costa, Monica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Lab Conservat Physiol LaFisC, Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Grad Program Biotechnol & Environm Monitoring, Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Lab Struct & Funct Biol LaBEF, Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, Lab Biochem & Microbiol LaBioM, Sao Carlos, SP - Brazil

^[5] Univ Estadual Campinas, Lab Nat Hist Brazilian Amphibians LaHNAB, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 207, JAN 1 2021.
• Citações Web of Science: 2

Resumo

To regulate the presence of contaminants in Brazilian water, the Brazilian Environmental Council (CONAMA) promulgates regulations regarding the concentrations of given compounds that are supposed to be safe for aquatic life. Considering these regulations, this study tested the effects of considered safe levels of lithium (2.5 mgL(-1)) and selenium (0.01 mgL(-1)), isolated and mixed, on the American bullfrog (Lithobates catesbeianus) tadpoles. The evaluation was done through the use of biomarkers of larval development as total wet weight (TWW), snout-vent-length (SVL), hind-limb-length (HLL), activity level (AL), histologic evaluation of the thyroid gland and the mortality rate. The tadpoles were allocated into four groups (n = 20 each): a control group (CT); a group exposed to lithium (LI), a group exposed to selenium (SE), and a group exposed to both lithium and selenium (SELI). The whole assay was carried out over 21 days, with two rounds of data collection (on 7th and 21st day) to evaluate the responses over time. A statistical reduction in the AL was observed in the tadpoles from the LI and SELI groups after 7 days of exposure, the same pattern was observed after 21 days. Histological analyses of the thyroid gland showed signs of up-regulation (i.e. statistic reduction in number and area of the follicles, as well a significant reduction in the area of the gland) in all exposed groups, which represents an endocrine response as an adaptative strategy to deal with polluted aquatic environment. The stress triggered by the polluted medium is discussed. (AU)

• Processo FAPESP: 17/03653-6 - Sixth International Conference on Environmental Management, Engineering, Planning and Economics (CEMEPE) and to the SECOTOX
• Beneficiário: Fábio Camargo Abdalla
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 08/57712-4 - Instituto Nacional de Pesquisas em Fisiologia Comparada
• Beneficiário: Augusto Shinya Abe
• Modalidade de apoio: Auxílio à Pesquisa - Temático