Tear proteomic profile in three distinct ocular surface diseases: keratoconus, pterygium, and dry eye related to graft-versus-host disease

Autor(es)

de Almeida Borges, Daniel ^[1] ; Alborghetti, Marcos Rodrigo ^[2] ; Franco Paes Leme, Adriana ^[3] ; Ramos Domingues, Romenia ^[3] ; Duarte, Bruna ^[1] ; Veiga, Melina ^[1] ; Trindade Ferrer, Marilia ^[1] ; Viana Wanzeler, Ana Claudia ^[1] ; Leite Arieta, Carlos Eduardo ^[1] ; Alves, Monica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Fac Med Sci, Dept Ophthalmol & Otorhinolaryngol, Campinas, SP - Brazil

^[2] Univ Brasilia, Dept Cell Biol, Brasilia, DF - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL PROTEOMICS; v. 17, n. 1 DEC 2020.
• Citações Web of Science: 0

Resumo

Background Diseases of the anterior segment of the eye may present different mechanisms, intensity of symptoms, and impact on the patients' quality of life and vision. The tear film is in direct contact with the ocular surface and cornea and can be easily accessed for sample collection, figuring as a promising source of potential biomarkers for diagnosis and treatment control. This study aimed to evaluate tear proteomic profile in 3 distinct ocular diseases: keratoconus (corneal ectasia), severe dry eye related to graft-versus-host-disease (tear film dysfunction and ocular inflammatory condition) and pterygium (conjunctival fibrovascular degenerative disease). Methods Tear samples were collected from patients of each condition and a control group. By using mass spectrometric analysis combined with statistics and bioinformatics tools, a detailed comparison of protein profile was performed. Results After Student's t-test analyses comparing each condition to the control group, we found the following number of differentially expressed proteins: 7 in keratoconus group, 29 in pterygium group, and 79 in GVHD group. Following multivariate analyses, we also report potential candidates as biomarkers for each disease. Conclusions We demonstrated herein that mass spectrometry-based proteomics was able to indicate proteins that differentiate three distinct ocular conditions, which is a promising tool for the diagnosis of ocular diseases. (AU)

• Processo FAPESP: 14/19138-5 - Criação do biobanco de tecidos oculares e investigação de novos mecanismos fisiopatológicos relacionados a doenças oculares do segmento anterior
• Beneficiário: Mônica de Cássia Alves
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores