Cellular and apoptotic status monitoring according to the ability and speed to resume post-cryopreservation embryonic development

Autor(es)

Valente, Roniele Santana ^[1] ; de Almeida, Tamie Guibu ^[2] ; Alves, Mayra Fernanda ^[2] ; Paschoal, Daniela Martins ^[3] ; Basso, Andrea Cristina ^[2] ; Sudano, Mateus Jose ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil

^[2] Vitro Brasil ABS Pecplan, Mogi Mirim, SP - Brazil

^[3] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Genet & Evolut, Rod. Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 158, p. 290-296, DEC 2020.
• Citações Web of Science: 0

Resumo

Embryonic morphofunctional competence features regulating post-cryopreservation resumption of development are still poorly understood. In this study, we investigated the correlation between embryonic viability and the speed and ability to resume post-cryopreservation development. Thus, in vitro produced blastocysts were vitrified by the Cryotop method using standard protocols. Subsequently, the embryos were warmed, re-cultured, and classified into groups according to their speed and ability to resume post-cryopreservation development: embryos not re-expanded at 12h (NE12); embryos reexpanded at 12h and hatched at 24h (E12H24); embryos re-expanded at 12h and hatched at 48h (E12H48); embryos re-expanded at 12h and not hatched at 48h (E12NH48). Subsequently, the embryos were subjected to monitoring of total cell number and apoptosis. We identified that the blastocoel's ability to re-expand was negatively affected by the significant higher percentage of apoptotic cells observed in the NE12 group than in the other groups. A greater (P < 0.05) number of total cells, found in groups E12H24 and E12H48, seems to have a positive influence on the hatching capacity of blastocysts after cryopreservation. In conclusion, the total number of cells and apoptotic index correlated with the speed and ability to resume post-cryopreservation development. Apoptosis was a determinant for embryonic re-expansion, and the total cell number was crucial for blastocyst hatching. (C) 2020 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 19/16239-9 - Modulação da acil-coa sintetase de cadeia longa sobre o desenvolvimento, depósito lipídico, e criotolerância de embriões bovinos produzidos in vitro
• Beneficiário: Mateus José Sudano
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo