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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cellular and apoptotic status monitoring according to the ability and speed to resume post-cryopreservation embryonic development

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Author(s):
Valente, Roniele Santana [1] ; de Almeida, Tamie Guibu [2] ; Alves, Mayra Fernanda [2] ; Paschoal, Daniela Martins [3] ; Basso, Andrea Cristina [2] ; Sudano, Mateus Jose [1, 4]
Total Authors: 6
Affiliation:
[1] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil
[2] Vitro Brasil ABS Pecplan, Mogi Mirim, SP - Brazil
[3] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Genet & Evolut, Rod. Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Theriogenology; v. 158, p. 290-296, DEC 2020.
Web of Science Citations: 0
Abstract

Embryonic morphofunctional competence features regulating post-cryopreservation resumption of development are still poorly understood. In this study, we investigated the correlation between embryonic viability and the speed and ability to resume post-cryopreservation development. Thus, in vitro produced blastocysts were vitrified by the Cryotop method using standard protocols. Subsequently, the embryos were warmed, re-cultured, and classified into groups according to their speed and ability to resume post-cryopreservation development: embryos not re-expanded at 12h (NE12); embryos reexpanded at 12h and hatched at 24h (E12H24); embryos re-expanded at 12h and hatched at 48h (E12H48); embryos re-expanded at 12h and not hatched at 48h (E12NH48). Subsequently, the embryos were subjected to monitoring of total cell number and apoptosis. We identified that the blastocoel's ability to re-expand was negatively affected by the significant higher percentage of apoptotic cells observed in the NE12 group than in the other groups. A greater (P < 0.05) number of total cells, found in groups E12H24 and E12H48, seems to have a positive influence on the hatching capacity of blastocysts after cryopreservation. In conclusion, the total number of cells and apoptotic index correlated with the speed and ability to resume post-cryopreservation development. Apoptosis was a determinant for embryonic re-expansion, and the total cell number was crucial for blastocyst hatching. (C) 2020 Elsevier Inc. All rights reserved. (AU)