High throughput fluorimetric assessment of iron traffic and chelation in iron-overloadedCaenorhabditis elegans

Autor(es)

Esposito, Breno Pannia ^[1] ; Martins, Airton Cunha ^[2] ; de Carvalho, Rodrigo Rodrigues Victor ^[1] ; Aschner, Michael ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Paulo, SP - Brazil

^[2] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10467 - USA

• Tipo de documento: Artigo Científico
• Fonte: BIOMETALS; v. 33, n. 4-5, p. 255-267, OCT 2020.
• Citações Web of Science: 1

Resumo

The nematodeCaenorhabditis elegans(C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2)C. elegansnematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases. (AU)

• Processo FAPESP: 18/19684-0 - Aplicações de novos derivados de desferrioxamina com alta permeabilidade celular
• Beneficiário: Breno Pannia Esposito
• Modalidade de apoio: Auxílio à Pesquisa - Regular