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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High throughput fluorimetric assessment of iron traffic and chelation in iron-overloadedCaenorhabditis elegans

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Author(s):
Esposito, Breno Pannia [1] ; Martins, Airton Cunha [2] ; de Carvalho, Rodrigo Rodrigues Victor [1] ; Aschner, Michael [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Sao Paulo, SP - Brazil
[2] Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10467 - USA
Total Affiliations: 2
Document type: Journal article
Source: BIOMETALS; v. 33, n. 4-5, p. 255-267, OCT 2020.
Web of Science Citations: 1
Abstract

The nematodeCaenorhabditis elegans(C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2)C. elegansnematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases. (AU)

FAPESP's process: 18/19684-0 - Applications of new desferrioxamine derivatives with high cell permeability
Grantee:Breno Pannia Esposito
Support Opportunities: Regular Research Grants