Overcoming matrix effects in the analysis of pyrethroids in honey by a fully automated direct immersion solid-phase microextraction method using a matrix-compatible fiber

Autor(es)

Belinato, Joao Raul ^{[1, 2, 3]} ; Grandy, Jonathan J. ^[3] ; Khaled, Abir ^[3] ; Suarez, Paola Alejandra Ortiz ^[3] ; Pawliszyn, Janusz ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Natl Inst Sci & Technol Bioanal INCTBio, Campinas, SP - Brazil

^[3] Univ Waterloo, Dept Chem, 200 Univ Ave West, Waterloo, ON N2L 3G1 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 340, MAR 15 2021.
• Citações Web of Science: 0

Resumo

Pyrethroids insecticides may constitute a major hazard to honeybees, leading to colony collapse disorder. However, the determination of pyrethroids in honey has remained a challenging undertaking for analysts to date due to the high complexity of this matrix as well as the MRLs. This paper presents a fully automated method to overcome matrix influences using matrix-compatible overcoated SPME fiber for quantitative analysis of pyrethroids in diluted honey by GC-MS. The developed method was optimized using a multivariate approach providing LOQ values much lower than the stablished MRL (0.10-10 ng/g), while granting satisfactory linearity (R-2 > 0.998) in a wide linear range of 0.1-2000 ng/g, repeatability with RSDs < 10%, reproducibility RSDs < 20%, and accuracy ranging from 75 to 118% and from 82 to 120 % for inter-day and intra-day assays, respectively by using five replicates. The method herein proposed overcomes challenges presented by complex matrices while minimizing sample handling and the overall complexity of the procedure. (AU)

• Processo FAPESP: 18/25729-7 - Revestimentos SPME biocompatíveis de alto rendimento para análises metabolômicas
• Beneficiário: Joao Raul Belinato de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/20547-2 - Caracterização do metaboloma de microorganismos anti-fitopatógenos por Cromatografia Gasosa Multidimensional e Espectrometria de Massas Simples e em Tandem(GC×GC-QMS e GC×GC-MS/MS)
• Beneficiário: Joao Raul Belinato de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado