Phenotype evaluation of human and canine isolates of Leishmania infantum

Autor(es)

Araujo, Thalita Fernanda ^[1] ; Calarga, Aline Parolin ^[2] ; Zorgi, Nahiara Esteves ^[1] ; Rangel, Osias ^[3] ; Levy, Carlos Emilio ^[4] ; Giorgio, Selma ^[1]

Afiliação do(s) autor(es)

^[1] State Campinas Univ, Biol Inst, Dept Anim Biol, Campinas, SP - Brazil

^[2] State Campinas Univ, Biol Inst, Dept Genet & Evolut Microbiol & Immunol, Campinas, SP - Brazil

^[3] Superintendencia Controle Endemias, Secao Tecn Pesquisa Controle DeVetores, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Fac Med Sci, Dept Clin Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 73, DEC 2020.
• Citações Web of Science: 0

Resumo

Human visceral leishmaniasis (VL) and canine leishmaniasis (CanL) in countries of South and Central America are caused by Leishmania infantum and has been endemic in Brazil for several years. The parasite biodiversity as well as the pharmacologic properties of drugs and the host species, are involved in the efficacy or inefficacy of leishmaniasis treatments. Although there are substantial number of reports describing the genetic characterization of the clinical field isolates of L. infantum,the phenotypic parameters have been less studied. In this study isolates from human and canine leishmaniasis (Hum1 and Can1) obtained in Campinas, Sao Paulo state, Brazil were identified as L. infantum. The Hum1 and Can1 isolates exhibited typical promastigote growth pattern. Regarding morphological features Can1 isolate differed in cell size. The infectivity in vitro of both isolatesis lower compared to the reference strain of L. infantum. Moreover, the in vivo infectivity of the three parasites is similar in Balb/c mice. The Hum1 isolate is more sensitive to leishmanial drugs (amphotericin B, miltefosine and glucantime) than the Can1 isolate when inside human macrophages, but not when inside canine macrophages. These findings indicated that L. infantum isolates differs in some phenotypic characteristics. (AU)

• Processo FAPESP: 18/23302-6 - Leishmanioses de importância médica no Brasil: estudo de sistemas celulares e isolados clínicos de Leishmania infantum e L. braziliensis e avaliação de candidatos vacinais contra L. infantum
• Beneficiário: Selma Giorgio
• Modalidade de apoio: Auxílio à Pesquisa - Regular