Antimicrobial blue light and photodynamic therapy ... - BV FAPESP
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Antimicrobial blue light and photodynamic therapy inhibit clinically relevant 8-lactamases with extended-spectrum (ESBL) and carbapenemase activity

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Autor(es):
dos Anjos, Carolina [1] ; Sellera, Fabio P. [1] ; Ribeiro, Martha S. [2] ; Baptista, Mauricio S. [3] ; Pogliani, Fabio C. [1] ; Lincopan, Nilton [4, 5] ; Sabino, Caetano P. [6, 5]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil
[2] Natl Commiss Nucl Energy, Ctr Lasers & Applicat, Nucl & Energy Res Inst, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Dept Biochem, Inst Chem, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo - Brazil
[5] Univ Sao Paulo, Dept Clin Anal, Fac Pharmaceut Sci, Sao Paulo - Brazil
[6] BioLambda Sci & Commercial LTD, Sao Paulo, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 32, DEC 2020.
Citações Web of Science: 0
Resumo

Introduction: The production of beta-lactamases by Gram-negative bacteria is among the most important factors of resistance to antibiotics, which has contributed to therapeutic failures that currently threaten human and veterinary medicine worldwide. Antimicrobial photodynamic therapy and antimicrobial blue light have a broadspectrum antibacterial activity against multidrug-resistant and hypervirulent pathogens. Objective: To investigate the ability of antimicrobial blue light to inhibit the hydrolytic activity of clinically relevant beta-lactamase enzymes (i.e., KPC, IMP, OXA, CTX-M, and SHV), with further comparison of the inhibitory effects of antimicrobial blue light with methylene blue-mediated antimicrobial photodynamic therapy. Methods: Blue LED light (A = 410 +/- 10 nm) alone or red LED light (A = 660 +/- 10 nm) in combination with methylene blue were used to inactivate, in vitro, suspensions of Klebsiella pneumoniae strains producing clinically important beta-lactamase enzymes assigned to the A, B and D Ambler molecular classes. Furthermore, beta-lactamase activity inhibition mediated by antimicrobial blue light and methylene blue-mediated antimicrobial photodynamic therapy was measured by using the chromogenic beta-lactam substrate nitrocefin. Results: beta-lactamase activities were effectively inactivated by both visible light-based approaches. In this regard, antimicrobial blue light and methylene blue-antimicrobial photodynamic therapy led to a significant reduction in the hydrolysis of nitrocefin (81-98 %). Conclusion: Sublethal doses of antimicrobial blue light and methylene blue-mediated antimicrobial photodynamic therapy are equally effective to inhibit clinically significant beta-lactamases, including extended-spectrum beta-lactamases and carbapenemases. (AU)

Processo FAPESP: 16/25095-2 - Fotoinativação bacteriana de patógenos da mastite por meio da luz azul: mecanismos de ação e segurança celular - estudo pré clínico
Beneficiário:Carolina dos Anjos
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 19/10851-4 - Atividade sinérgica da luz azul em combinação com agentes antimicrobianos contra patógenos resistentes
Beneficiário:Carolina dos Anjos
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 17/22406-0 - Desenvolvimento de equipamento inteligente para aplicação clínica de fototerapias com ajuste dosimétrico automático e pagamento sob demanda
Beneficiário:Caetano Padial Sabino
Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE