In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus

Autor(es)

de Oliveira, Maria Eduarda B. S. ^[1] ; Sartoratto, Adilson ^[2] ; Cardoso, Jean Carlos ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos CCA UFSCar, Biotechnol Undergrad Course, Ctr Ciencias Agr, Rodovia Anhanguera, Km 174, BR-13600970 Araras - Brazil

^[2] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr CP, BR-13083970 Campinas - Brazil

^[3] CCA UFSCar, Dept Biotechnol Plant & Anim Prod, Rodovia Anhanguera, Km 174, BR-13600970 Araras - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 25, n. 24 DEC 2020.
• Citações Web of Science: 0

Resumo

The efficient production of plant-derived medicinal compounds (PDMCs) from in vitro plants requires improvements in knowledge about control of plant or organ development and factors affecting the biosynthesis pathway of specific PDMCs under in vitro conditions, leading to a realistic large-scale tool for in vitro secondary metabolite production. Thus, this study aimed to develop an in vitro technique, through the induction and proliferation of calli, for production of plant fresh weight, and to compare the PDMC profile obtained from the plants versus in vitro calli of Phyllanthus amarus. It was successfully possible to obtain and proliferate two types of calli, one with a beige color and a friable appearance, obtained in the dark using Murashige and Skoog (MS) medium plus 2,4-dichlorophenoxyacetic acid (2,4-D), and a second type with a green color, rigid consistency, and nonfriable appearance obtained under light conditions and MS medium plus 6-benzyladenine (6-BA). In vitro micropropagated plants that gave rise to calli were also acclimatized in a greenhouse and cultivated until obtaining the mass for PDMC analysis and used as a control. While the micropropagated-derived plants concentrated the lignans niranthin, nirtetralin, and phyllanthin, the Phyllanthus amarus calli proliferated in vitro concentrated a completely different biochemical profile and synthesis of compounds, such as betulone, squalene, stigmasterol, and beta-sitosterol, in addition to others not identified by GC-MS database. These results demonstrate the possibility of applying the calli in vitro from Phyllanthus amarus for production of important PDMCs unlike those obtained in cultures of differentiated tissues from field plants. (AU)

• Processo FAPESP: 19/00243-7 - Calogênese in vitro como alternativa a produção de filantina e hipofilantina em Phyllantus amarus
• Beneficiário: Maria Eduarda Barboza Souza de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica