Performance of the main downstream operations on hyaluronic acid purification

Autor(es)

Domingos Cavalcanti, Andre Delano ^[1] ; de Melo, Alice Gomes ^[1] ; Moreira Ferreira, Bruno Armenio ^[1] ; Andrade Santana, Maria Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Dev Biotechnol, Microbial Proc Lab, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Process Biochemistry; v. 99, p. 160-170, DEC 2020.
• Citações Web of Science: 0

Resumo

Hyaluronic acid (HA), or hyaluronan, is a natural polyelectrolyte, ubiquitous in human tissues. Exogenous HA has been a valuable material due to its wide range of medical applications, such as in osteoarthritis treatment, ophthalmic surgery, adhesion prevention after surgeries and wound healing, as well as cosmetic applications. However, to ensure the physicochemical and biological properties, a purity near to 99 % is a primary requirement, aiming clinical applications. To achieve this goal, various downstream operations have been used, aiming HA concentration, separation and purification. Precipitation with organic solvents has been a common operation in most purification processes, combined with other downstream operations such as precipitation with quaternary salts, filtration, adsorption and ion exchange. This work presents an updated review of HA purification, emphasizing the performance of the main downstream operations used to achieve highly purified HA, in the period from 1970 to 2019. We conclude that, in the majority of the published works, there is a lack of studies regarding the operational conditions, as well as an absence of the purification percentage development during the processes. (AU)

• Processo FAPESP: 15/23134-8 - Influência da associação entre plasma-rico em plaquetas e ácido hialurônico na liberação de fatores de crescimento, proliferação celular e diferenciação osteogênica/condrogênica
• Beneficiário: Bruna Alice Gomes de Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado