Hybrid microgels produced via droplet microfluidics for sustainable delivery of hydrophobic and hydrophilic model nanocarriers

Autor(es)

de Carvalho, Bruna Gregatti ^[1] ; Taketa, Thiago Bezerra ^[1] ; Moreno Garcia, Bianca Bonetto ^[2] ; Han, Sang Won ^[2] ; de la Torre, Lucimara Gaziola ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Chem Engn, Dept Mat & Bioproc Engn, BR-13083970 Campinas - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Ctr Cell Therapy & Mol, BR-04044010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 118, JAN 2021.
• Citações Web of Science: 0

Resumo

Drug delivery for treatment of chronic diseases relies on the effective delivery of payload materials into the target cells in a long-term release. In this context, the present study investigated hybrid microgels as platforms to carry nanoparticles to drug delivery. Hybrid microgels were produced with silk fibroin (SF) and chondroitin sulfate (CS), and alginate (ALG) by droplet microfluidics. ALG/SF, ALG/CS, and ALG/CS/SF microgels, ranging from 70-90 mu m, were tested to encapsulate two model nanoparticles, polystyrene latex beads in pristine form (NPs) and NPs coated with bovine serum albumin (NPs-BSA) to simulate hydrophobic and hydrophilic nano carriers, respectively. IR spectroscopy and fluorescence microscopy analysis confirmed the presence of SF and CS within ALG-based microgels revealing marked differences in their morphology and physicochemical properties. The release profiles of model nanoparticles revealed to be dependent on microgels composition and physicochemical properties. These findings show that SF ternary hybrid microgels facilitated the entrapment of hydrophobic nanocarriers with encapsulation efficiency (EE) from 83 to 98% keeping a better sustainable profile release than nonhybrid ALG microgels. Besides, CS improved the carriage of NPs-BSA (EE = 85%) and their profile release. The results highlight the versatility and tunable properties of these biobased microgels, being a good strategy to be used as an efficient platform in using macro and nanoencapsulated systems for drug delivery. (AU)

• Processo FAPESP: 18/19537-8 - Microfluídica como plataforma tecnológica para nano & biotecnologia
• Beneficiário: Lucimara Gaziola de la Torre
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/20206-8 - Modulação de monócitos, macrófagos e pericitos pelos genes dos fatores estimuladores de colônia para tratamento de isquemia de membros em modelo murino
• Beneficiário: Sang Won Han
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/18523-3 - Síntese de micropartículas poliméricas via processo microfluídico de gotas para a liberação sustentada de vetores não virais aplicados em terapia gênica
• Beneficiário: Bruna Gregatti de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto