The importance of the assembling of DNA strands on the performance of electrochemical genosensors

Autor(es)

Batistuti, Marina R. ^[1] ; Bueno, Paulo R. ^[2] ; Mulato, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters, Dept Phys, USP, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Sao Paulo State Univ, Inst Chem, Dept Phys Chem, UNESP, BR-14800060 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 159, DEC 2020.
• Citações Web of Science: 0

Resumo

Electrochemical biosensors have been extensively studied due to their capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers. Impedimetric DNA hybridization sensors are based on tracking negative charge variation over the electrode surface owing to the target sequence of hybridization to the single-stranded immobilized DNA probes. However, the development of this platform requires an understanding of how to control the immobilization and the structure formed on the interface. The DNA assembling, as is the case of the immobilization of the single-stranded DNA on the electrode surface and the subsequent hybridization, is crucial for the performance of the biosensor and is dependent on the solution environment characteristics, as we evidenced here. Particularly, the influence of DNA probe preparation and immobilization on gold surfaces were investigated using different protocols. The influence of negatively charged redox couple in solution was studied by impedance spectroscopy and we succeed in obtaining stable and reproducible results using target sequences from 10 pmol L-1 to 1 mmol L-1. We also verified that the ionic strength of the buffer has a strong influence over the immobilization process and sensor performance. (AU)

• Processo FAPESP: 17/24839-0 - Eletrônica e eletroquímica em escala nanométrica: fundamentos e aplicações
• Beneficiário: Paulo Roberto Bueno
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/14403-5 - Detecção de alterações relacionadas ao câncer de mama através de sensores de carga ou de massa acoplados a monocamadas de DNA
• Beneficiário: Marina Ribeiro Batistuti Sawazaki
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/09562-4 - Biossensores microeletrônicos associados a técnicas eletroquímicas
• Beneficiário: Marcelo Mulato
• Modalidade de apoio: Auxílio à Pesquisa - Regular