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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The importance of the assembling of DNA strands on the performance of electrochemical genosensors

Full text
Author(s):
Batistuti, Marina R. [1] ; Bueno, Paulo R. [2] ; Mulato, Marcelo [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters, Dept Phys, USP, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Sao Paulo State Univ, Inst Chem, Dept Phys Chem, UNESP, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Microchemical Journal; v. 159, DEC 2020.
Web of Science Citations: 0
Abstract

Electrochemical biosensors have been extensively studied due to their capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers. Impedimetric DNA hybridization sensors are based on tracking negative charge variation over the electrode surface owing to the target sequence of hybridization to the single-stranded immobilized DNA probes. However, the development of this platform requires an understanding of how to control the immobilization and the structure formed on the interface. The DNA assembling, as is the case of the immobilization of the single-stranded DNA on the electrode surface and the subsequent hybridization, is crucial for the performance of the biosensor and is dependent on the solution environment characteristics, as we evidenced here. Particularly, the influence of DNA probe preparation and immobilization on gold surfaces were investigated using different protocols. The influence of negatively charged redox couple in solution was studied by impedance spectroscopy and we succeed in obtaining stable and reproducible results using target sequences from 10 pmol L-1 to 1 mmol L-1. We also verified that the ionic strength of the buffer has a strong influence over the immobilization process and sensor performance. (AU)

FAPESP's process: 17/24839-0 - Nanoelectronics and nanoscale electrochemistry: fundaments and applications
Grantee:Paulo Roberto Bueno
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/14403-5 - Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers
Grantee:Marina Ribeiro Batistuti Sawazaki
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/09562-4 - Microelectronic biossensors associated with electrochemical techniques
Grantee:Marcelo Mulato
Support Opportunities: Regular Research Grants