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Design and fabrication of nanostructured flexible devices for biomarkers detection

Grant number: 16/01919-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2016
Effective date (End): August 31, 2021
Field of knowledge:Physical Sciences and Mathematics - Physics - Condensed Matter Physics
Principal researcher:Osvaldo Novais de Oliveira Junior
Grantee:Paulo Augusto Raymundo Pereira
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
Associated scholarship(s):17/26666-6 - Flexible sensors for detecting biomarkers with non-invasive procedures, BE.EP.PD


This Project is aimed at developing and characterizing flexible devices made of gold nanostructures obtained via photolithography on a platinum surface. These nanostructured flexible surfaces will be characterized by electrochemical techniques (cyclic voltammetry, electrochemical impedance spectroscopy), in addition to atomic force microscopy (AFM) and scanning electron microscopy (SEM), to determine the size and distribution of the gold nanostructures as well as the exposed area of platinum. With the considerable increase in area of the electroactive surfaces, the electrocatalytic activity of the nanostructured flexible device should be sufficient to detect biomarkers, as well as uric acid, ascorbic acid, dopamine and NADH. In a second step, the gold nanostructures will be coated with self-assembed monolayers (SAMs) of alkanethiol (mercaptoacetic acid and 11-mercaptoundecanoic) to prepare the surface for immobilization of biomolecules, such as enzymes, antibodies and DNA. This coating will be analyzed using electrochemical techniques (voltammetry, amperometry, electrochemical impedance spectroscopy), and infrared reflection-absorption with modulated polarization (PM-IRRAS). Detection will be conducted with electrochemical techniques in standard solutions. The biosensors will also be used for detecting lactate and cholesterol in sweat by applying the flexible devices directly onto the skin during physical activities, while detection of glucose and cortisol will be performed in saliva, thus avoiding invasive methods. The immunosensors and genosensors will be used for detection of head and neck cancer at early stages. (AU)

Articles published in Pesquisa para Inovação FAPESP about the scholarships:
Scientists at the University of São Paulo create a glove that detects pesticides in food 
News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications (18)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMARGO, ESSICA ROCHA; BACCARIN, MARINA; RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; OLIVEIRA, GEISER G.; FATIBELLO-FILHO, ORLANDO; OLIVEIRA, JR., OSVALDO N.; JANEGITZ, BRUNO C.. Electrochemical biosensor made with tyrosinase immobilized in a matrix of nanodiamonds and potato starch for detecting phenolic compounds. Analytica Chimica Acta, v. 1034, p. 137-143, . (15/19099-2, 16/01919-6, 13/14262-7)
MARTINS, THIAGO S.; BOTT-NETO, JOSE L.; RAYMUNDO-PEREIRA, PAULO A.; TICIANELLI, EDSON A.; MACHADO, SERGIO A. S.. An electrochemical furosemide sensor based on pencil graphite surface modified with polymer film Ni-salen and Ni(OH)(2)/C nanoparticles. SENSORS AND ACTUATORS B-CHEMICAL, v. 276, p. 378-387, . (13/16930-7, 16/01919-6, 16/12759-0)
RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; VICENTINI, FERNANDO C.; JANEGITZ, BRUNO C.; MENDONCA, CAMILA D.; FURINI, LEONARDO N.; BOAS, NAIZA V.; CALEGARO, MARCELO L.; CONSTANTINO, CARLOS J. L.; MACHADO, SERGIO A. S.; et al. Sensitive detection of estriol hormone in creek water using a sensor platform based on carbon black and silver nanoparticles. Talanta, v. 174, p. 652-659, . (13/14262-7, 15/19099-2, 16/01919-6, 14/05197-0, 16/12759-0, 12/17689-9)
RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; MENDONCA, CAMILA D.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Printex 6L Carbon Nanoballs used in Electrochemical Sensors for Simultaneous Detection of Emerging Pollutants Hydroquinone and Paracetamol. SENSORS AND ACTUATORS B-CHEMICAL, v. 252, p. 165-174, . (13/14262-7, 16/01919-6, 14/05197-0, 16/12759-0, 12/17689-9)
CAMPOS, ANDERSON M.; RAYMUNDO-PEREIRA, PAULO A.; MENDONCA, CAMILA D.; CALEGARO, MARCELO L.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Size Control of Carbon Spherical Shells for Sensitive Detection of Paracetamol in Sweat, Saliva, and Urine. ACS APPLIED NANO MATERIALS, v. 1, n. 2, p. 654-661, . (13/14262-7, 16/01919-6, 14/05197-0, 16/12759-0, 12/17689-9)
RAYMUNDO-PEREIRA, PAULO A.; SILVA, TIAGO A.; CAETANO, FABIO R.; RIBOVSKI, LAIS; ZAPP, EDUARDO; BRONDANI, DANIELA; BERGAMINI, MARCIO F.; MARCOLINO, JR., LUIZ H.; BANKS, CRAIG E.; OLIVEIRA, JR., OSVALDO N.; et al. Polyphenol oxidase-based electrochemical biosensors: A review. Analytica Chimica Acta, v. 1139, p. 198-221, . (16/01919-6, 20/01050-5, 18/22214-6, 17/21097-3, 17/21898-6)
PEREIRA, TAMIRES DOS SANTOS; MAURUTO DE OLIVEIRA, GABRIELA C.; SANTOS, FABRICIO APARECIDO; RAYMUNDO-PEREIRA, PAULO A.; OLIVEIRA, JR., OSVALDO N.; JANEGITZ, BRUNO CAMPOS. Use of zein microspheres to anchor carbon black and hemoglobin in electrochemical biosensors to detect hydrogen peroxide in cosmetic products, food and biological fluids. Talanta, v. 194, p. 737-744, . (16/18270-2, 13/14262-7, 15/19099-2, 16/01919-6, 17/17572-8, 16/04743-6)
RAYMUNDO-PEREIRA, PAULO A.; BACCARIN, MARINA; OLIVEIRA, JR., OSVALDO N.; JANEGITZ, BRUNO C.. Thin Films and Composites Based on Graphene for Electrochemical Detection of Biologically-relevant Molecules. Electroanalysis, v. 30, n. 9, p. 1888-1896, . (16/01919-6, 13/14262-7, 17/21898-6)
DELGADO, KARINA P.; RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; OLIVEIRA, JR., OSVALDO N.; JANEGITZ, BRUNO C.. Ultralow Cost Electrochemical Sensor Made of Potato Starch and Carbon Black Nanoballs to Detect Tetracycline in Waters and Milk. Electroanalysis, v. 30, n. 9, p. 2153-2159, . (13/14262-7, 15/19099-2, 16/19885-0, 16/01919-6)
RAYMUNDO-PEREIRA, PAULO A.; GOMES, NATHALIA O.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Simultaneous, ultrasensitive detection of hydroquinone, paracetamol and estradiol for quality control of tap water with a simple electrochemical method. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 848, . (16/12759-0, 13/14262-7, 16/01919-6, 19/01777-5)
SA, ACELINO C. DE; BARBOSA, SIMONE C.; RAYMUNDO-PEREIRA, PAULO A.; WILSON, DEIVY; SHIMIZU, FLAVIO M.; RAPOSO, MARIA; OLIVEIRA, JR., OSVALDO N.. Flexible Carbon Electrodes for Electrochemical Detection of Bisphenol-A, Hydroquinone and Catechol in Water Samples. CHEMOSENSORS, v. 8, n. 4, . (18/22214-6, 16/01919-6)
ROMANHOLO, PEDRO V. V.; RAZZINO, CLAUDIA A.; RAYMUNDO-PEREIRA, PAULO A.; PRADO, THIAGO M.; MACHADO, SERGIO A. S.; SGOBBI, LIVIA F.. Biomimetic electrochemical sensors: New horizons and challenges in biosensing applications. BIOSENSORS & BIOELECTRONICS, v. 185, . (16/01919-6)
CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; et al. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, . (17/03503-4, 12/15543-7, 18/02819-0, 15/12990-0, 13/14262-7, 19/13390-8, 16/01919-6)
SILVA, ROBSON R.; RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; WILSON, DEIVY; OTONI, CAIO G.; BARUD, HERNANE S.; COSTA, CARLOS A. R.; DOMENEGUETTI, RAFAEL R.; BALOGH, DEBORA T.; RIBEIRO, SIDNEY J. L.; et al. Microbial nanocellulose adherent to human skin used in electrochemical sensors to detect metal ions and biomarkers in sweat. Talanta, v. 218, . (16/06612-6, 15/01770-0, 16/01919-6, 13/14262-7)
SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, v. 5, n. 15, p. 5658-5670, . (19/07811-0, 18/19750-3, 20/02938-0, 16/01919-6, 18/18953-8, 19/13514-9, 14/50867-3, 16/23763-8, 18/22214-6, 19/00101-8)
RAYMUNDO-PEREIRA, PAULO A.; GOMES, NATHALIA O.; SHIMIZU, FLAVIO M.; MACHADO, SERGIO A. S.; OLIVEIRA, JR., OSVALDO N.. Selective and sensitive multiplexed detection of pesticides in food samples using wearable, flexible glove-embedded non-enzymatic sensors. CHEMICAL ENGINEERING JOURNAL, v. 408, . (16/01919-6, 19/01777-5, 18/22214-6, 13/14262-7)
RAYMUNDO-PEREIRA, PAULO A.; PEDRO, RAFAEL DE OLIVEIRA; CARR, OLIVIA; MELENDEZ, MATIAS E.; GOBBI, ANGELO L.; PIAZZETTA, MARIA HELENA DE OLIVEIRA; CARVALHO, ANDRE L.; REIS, RUI M.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N.. Influence of the Molecular Orientation and Ionization of Self-Assembled Monolayers in Biosensors: Application to Genosensors of Prostate Cancer Antigen 3. Journal of Physical Chemistry C, v. 125, n. 1, p. 498-506, . (16/01919-6, 17/03503-4, 18/22214-6, 18/02819-0)
RAYMUNDO-PEREIRA, PAULO A.; CECCATO, DIEGO A.; JUNIOR, AIRTON G. B.; TEIXEIRA, MARCOS F. S.; LIMA, SERGIO A. M.; PIRES, ANA. M.. Study on the structural and electrocatalytic properties of Ba2+- and Eu3+-doped silica xerogels as sensory platforms. RSC ADVANCES, v. 6, n. 106, p. 104529-104536, . (15/10394-1, 12/17689-9, 08/07298-7, 16/01919-6)

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Filed patent(s) as a result of this research project