Conjugation of superparamagnetic iron oxide nanoparticles and curcumin photosensitizer to assist in photodynamic therapy

Autor(es)

de Santana, Willian Max O. S. ^[1] ; Caetano, Bruno L. ^{[2, 3, 4]} ; de Annunzio, Sarah Raquel ^[2] ; Pulcinelli, Sandra H. ^[1] ; Menager, Christine ^[4] ; Fontana, Carla Raquel ^[2] ; Santilli, V, Celso

Afiliação do(s) autor(es)

^[1] V, Sao Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[3] Sorbonne Univ, PSL Univ, CNRS, ESPCI Paris, Soft Matter Sci & Engn, F-75005 Paris - France

^[4] Sorbonne Univ, CNRS, PHENIX, Physicochim Electrolytes & Nanosyst Interfaciaux, F-75005 Paris - France

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 196, DEC 2020.
• Citações Web of Science: 0

Resumo

In this work, we describe the synthesis and characterization of the SPIONP-CUR conjugate between curcumin (CUR) and superparamagnetic iron oxide nanoparticles (SPIONPs), in addition to its application in photo-dynamic therapy (PDT) using a protocol free of organic solvents as a dispersant. The SPIONP-CUR conjugate was characterized by X-ray diffraction, transmission electron microscopy, zeta potential measurements, Fourier transform infrared spectroscopy, thermogravimetry, magnetometry and magnetic hyperthermia assays. The SPIONP-CUR conjugation occurred by bonding between the keto-enol moiety of CUR and the iron atoms present on the surfaces of the SPIONPs. The conjugate showed heating power under an alternating magnetic field (AMF) and photodynamic action when irradiated with blue LED light. In experiments using PDT against Staphylococcus aureus in the planktonic phase, it was demonstrated that with application of blue light at 3.12 J cm(-2), the conjugate (dispersed in water) caused a total reduction of the bacterial load. In the absence of light, the reduction was insignificant, even after 24 h of contact with the bacteria. (AU)

• Processo FAPESP: 18/23015-7 - Terapia fotodinâmica antimicrobiana via irradiação em modo contínuo e chaveado contra Enterococcus faecalis e Cutibacterium acnes
• Beneficiário: Carla Raquel Fontana
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/09088-1 - Avaliação da terapia fotodinâmica contra biofilmes acneicos polimicrobianos: um estudo pré-clínico
• Beneficiário: Sarah Raquel de Annunzio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/07822-7 - Conjugação de nanopartículas superparamagnéticas de óxido de ferro e curcumina: associação da terapia fotodinâmica e hipertermia magnética
• Beneficiário: Willian Max Oliveira de Souza de Santana
• Modalidade de apoio: Bolsas no Brasil - Mestrado