Increased Anxiety-Like Behavior in the Acute Phase of a Preclinical Model of Periodontal Disease

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Varotto, Bruna Luiza Roim ^{[1, 2]} ; Martinez, Raquel Chacon Ruiz ^{[3, 4]} ; Gouveia, Flavia Venetucci ^{[3, 5]} ; Antunes, Geiza Fernanda ^{[3, 4]} ; Fabri, Gisele Maria de Campos ^[6] ; Ballester, Gerson ^[7] ; Antequera, Reynaldo ^{[1, 2]} ; de Siqueira, Silvia Regina Dowgan Tesseroli ^{[8, 9]} ; Fonoff, Erich Talamoni ^{[8, 9]} ; Teixeira, Manoel Jacobsen ^{[8, 9]} ; de Siqueira, Jose Tadeu Tesseroli ^{[10, 8, 1, 2, 9]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dent Team, Inst Psychiat, Sch Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dent Res Grp Pain & Mental Hlth, Inst Psychiat, Sch Med, Sao Paulo - Brazil

^[3] Hosp Sirio Libanes, Div Neurosci, Sao Paulo - Brazil

^[4] Univ Sao Paulo, LIM 23 Inst Psychiat, Sch Med, Sao Paulo - Brazil

^[5] Sunnybrook Res Inst, Biol Sci Platform, Toronto, ON - Canada

^[6] Univ Juiz de Fora, Clin Dent Dept, Juiz De Fora - Brazil

^[7] Univ Cidade Sao Paulo, Sch Med, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Sch Med, Dept Neurol, Div Funct Neurosurg, Sao Paulo - Brazil

^[9] Univ Sao Paulo, Sch Med, Dept Neurol, Pain Ctr, Sao Paulo - Brazil

^[10] Univ Sao Paulo, Sch Med, Hosp Clin, Orofacial Pain Team, Dent Div, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN NEUROLOGY; v. 11, DEC 22 2020.
• Citações Web of Science: 0

Resumo

Periodontal disease (PD) is an infectious-inflammatory oral disease that is highly prevalent among adolescence and adulthood and can lead to chronic orofacial pain and be associated with anxiety, stress and depression. This study aimed to identify anxiety-like behaviors in the ligature-induced murine preclinical model of PD in different phases of the disease (i.e., acute vs. chronic). Also, we investigated orofacial mechanical allodynia thresholds and superficial cortical plasticity along the orofacial motor cortex in both disease phases. To this aim, 25 male Wistar rats were randomly allocated in acute (14 days) or chronic (28 days) ligature-induced-PD groups and further divided into active-PD or sham-PD. Anxiety-like behavior was evaluated using the elevated plus maze, mechanical allodynia assessed using the von Frey filaments test and superficial motor cortex mapping was performed with electrical transdural stimulation. We observed increased anxiety-like behavior in active-PD animals in the acute phase, characterized by decreased number of entries into the open arm extremities {[}t((1,7)) = 2.42, p = 0.04], and reduced time spent in the open arms {[}t((1,7)) = 3.56, p = 0.01] and in the open arm extremities {[}t((1,7)) = 2.75, p = 0.03]. There was also a reduction in the mechanical allodynia threshold in all active-PD animals {[}Acute: t((1,7)) = 8.81, p < 0.001; Chronic: t((1,6)) = 60.0, p < 0.001], that was positively correlated with anxiety-like behaviors in the acute group. No differences were observed in motor cortex mapping. Thus, our findings show the presence of anxiety-like behaviors in the acute phase of PD making this a suitable model to study the impact of anxiety in treatment response and treatment efficacy. (AU)

• Processo FAPESP: 11/08575-7 - Papel da modulação dopaminérgica no núcleo lateral da amígdala sobre a resposta condicionada de esquiva
• Beneficiário: Raquel Chacon Ruiz Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/20602-5 - Análise prospectiva dos resultados funcionais da amigdalotomia bilateral assistencial em pacientes com agressividade refratária
• Beneficiário: Flavia Venetucci Gouveia
• Modalidade de apoio: Bolsas no Brasil - Doutorado