Clinical influence of calcium hydroxide and N-acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis

Autor(es)

Corazza, B. J. M. ^[1] ; Martinho, F. C. ^[2] ; Khoury, R. D. ^[1] ; Toia, C. C. ^[1] ; Orozco, E. I. F. ^[1] ; Prado, R. F. ^[1] ; Machado, F. P. ^[1] ; Valera, M. C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Endodont Div, Dept Restorat Dent, Eng Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Maryland, Sch Dent, Dept Adv Oral Sci & Therapeut, Baltimore, MD 21201 - USA

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 54, n. 1, p. 61-73, JAN 2021.
• Citações Web of Science: 0

Resumo

Aim To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication {[}Ca(OH)(2)], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 in periapical tissues. Methodology Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: {[}Ca(OH)(2)] + saline solution (SSL) {[}Ca(OH)(2) + SSL group] (n = 12), Ca(OH)(2) + 2% chlorhexidine gel {[}Ca(OH)(2) + 2% CHX gel group] (n = 12) and NAC {[}NAC group] (n = 12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications(S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test and Kruskal-Wallis test, followed by Dunn'spost hoctest; all statistical tests were performed at a significance level of 5%. Results RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH)(2)medication did not increase the levels of RvE1 or RvD2 (bothP > 0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (P < 0.05). Conclusions RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment. (AU)

• Processo FAPESP: 16/26012-3 - Avaliação clínica do efeito de diferentes medicaçoes intracanais nos níveis de resolvinas e lipoxinas periapicais - novas perspecticas endodônticas
• Beneficiário: Bruna Jordão Motta Corazza
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/01703-9 - Infecção endodôntica primária versus secundária: perfil inflamatório e lipídico, perfil microbiano, níveis de endotoxinas e de ácido lipoteicóico antes e durante a terapia endodôntica e sucesso após 18 meses de tratamento.
• Beneficiário: Marcia Carneiro Valera Garakis
• Modalidade de apoio: Auxílio à Pesquisa - Regular