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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Investigation of microbial profile, levels of endotoxin and lipoteichoic acid in teeth with symptomatic irreversible pulpitis: a clinical study

Texto completo
Autor(es):
Arruda-Vasconcelos, R. [1, 2] ; Louzada, L. M. [1] ; Feres, M. [3] ; Tomson, P. L. [2] ; Cooper, P. R. [4] ; Gomes, B. P. F. A. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Div Endodont, Dept Restorat Dent, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Birmingham, Inst Clin Sci, Dept Oral Biol, Sch Dent, Birmingham, W Midlands - England
[3] Univ Guarulhos, Dent Res Div, Dept Periodontol, Guarulhos - Brazil
[4] Univ Otago, Sir John Walsh Res Inst, Dept Oral Sci, Fac Dent, Dunedin - New Zealand
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: International Endodontic Journal; v. 54, n. 1, p. 46-60, JAN 2021.
Citações Web of Science: 0
Resumo

Aim To investigate the microbial profile, and levels of endotoxin (LPS) and lipoteichoic acid (LTA), in infected dentine (ID) and root canals (RC) at different phases of root canal treatment in teeth with symptomatic irreversible pulpitis. Methodology Ten volunteers were included, and samples were collected from infected dentine (ID) and the root canal lumen (RC) using sterile excavators and paper points, respectively. RC samples were taken before (S1) and after (S2) chemo-mechanical canal preparation (CMP), and after intracanal medication (ICM; S3). Checkerboard DNA-DNA hybridization was used for microbial analysis. The levels of LPS and LTA were evaluated using the limulus amebocyte lysate assay and ELISA, respectively. Shapiro-Wilk's test was used to verify data normality. Friedman's test was used to evaluate statistical differences using checkerboard DNA-DNA hybridization in the ID and RC at the different phases of the RC treatment.Post hocDunn's multiple comparison test was used to verify significant differences recorded at the different time-points. The levels of LPS and LTA were analysed statistically by using repeated measuresanovaand Tukey'spost hoctest to evaluate differences in both sites. The significance level was set at 5% (P < 0.05). Results A total of 40 DNA probes were used for microbial investigation of ID and RC samples using checkerboard DNA-DNA hybridization. The levels and complexity of bacteria were similar in the ID and initial RC samples. The levels of LPS and LTA in ID were significantly higher than the initial RC samples (S1;P < 0.05). Canal preparation was effective in significantly decreasing the levels of bacteria, LPS and LTA (P < 0.05). ICM did not provide additional reduction in the levels of bacteria and LPS (P > 0.05). However, a significant reduction in the levels of LTA was observed after ICM (P < 0.05). Conclusion The microbial profile of infected dentine and root canals of teeth with irreversible pulpitis was complex, harbouring different species including Gram-positive and Gram-negative, cocci and bacilli, and facultative and strict anaerobes. Root canal preparation was effective in reducing the levels of bacteria, LPS and LTA from the root canals of teeth with pulpitis. (AU)

Processo FAPESP: 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
Beneficiário:Brenda Paula Figueiredo de Almeida Gomes
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 19/10755-5 - Investigação clínica de modulações epigenéticas em polpas dentais com diagnóstico de pulpite irreversível
Beneficiário:Rodrigo Arruda Vasconcelos
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
Processo FAPESP: 17/25242-8 - Monitoramento clínico, microbiológico e imunológico de pacientes submetidos à terapia endodôntica com diagnóstico de Pulpite irreversível
Beneficiário:Rodrigo Arruda Vasconcelos
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 19/19300-0 - Investigação dos aspectos clínicos, microbiológicos e imunológicos de dentes com diagnóstico de lesão periodontal primária com envolvimento endodôntico secundário
Beneficiário:Lidiane Mendes Louzada
Linha de fomento: Bolsas no Brasil - Doutorado