Design, synthesis, and evaluation of Bothrops venom serine protease peptidic inhibitors

Autor(es)

Gloria Maria da Silva ^[1] ; Daniel Henrique Berto de Souza ^[2] ; Karoline B. Waitman ^[3] ; Matteo Celano Ebram ^[4] ; Melissa R. Fessel ^[5] ; Iuliu Cezar Zainescu ^[6] ; Fernanda C. Portaro ^[7] ; Montse Heras ^[8] ; Sonia A. de Andrade ^[9]

Afiliação do(s) autor(es)

^[1] Butantan Institute. Laboratory of Pain and Signaling - Brasil

^[2] Butantan Institute. Laboratory of Pain and Signaling - Brasil

^[3] Butantan Institute. Laboratory of Pain and Signaling - Brasil

^[4] Butantan Institute. Laboratory of Pain and Signaling - Brasil

^[5] Butantan Institute. Laboratory of Molecular Biology - Brasil

^[6] University of Oxford. Department of Chemistry - Reino Unido

^[7] Butantan Institute. Laboratory of Immunochemistry - Brasil

^[8] University of Girona. Department of Chemistry. Laboratory of Innovation in Processes and Products of Organic Synthesis - Espanha

^[9] Butantan Institute. Laboratory of Pain and Signaling - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 27, 2021-01-15.

Resumo

Abstract Background: In Central and South America, snakebite envenomation is mainly caused by Bothrops spp. snakes, whose venoms feature significant biochemical richness, including serine proteases. The available bothropic antivenoms are efficient in avoiding fatalities, but do not completely neutralize venom serine proteases, which are co-responsible for some disorders observed during envenomation. Methods: In order to search for tools to improve the antivenom’s, 6-mer peptides were designed based on a specific substrate for Bothrops jararaca venom serine proteases, and then synthesized, with the intention to selectively inhibit these enzymes. Results: Using batroxobin as a snake venom serine protease model, two structurally similar inhibitor peptides were identified. When tested on B. jararaca venom, one of the new inhibitors displayed a good potential to inhibit the activity of the venom serine proteases. These inhibitors do not affect human serine proteases as human factor Xa and thrombin, due to their selectivity. Conclusion: Our study identified two small peptides able to inhibit bothropic serine proteases, but not human ones, can be used as tools to enhance knowledge of the venom composition and function. Moreover, one promising peptide (pepC) was identified that can be explored in the search for improving Bothrops spp. envenomation treatment. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/04321-7 - Síntese de inibidores peptídicos: uma estratégia para melhoria do soro antibotrópico produzido pelo Instituto Butantan
• Beneficiário: Sonia Aparecida de Andrade Chudzinski
• Modalidade de apoio: Auxílio à Pesquisa - Regular