Identification of hepatic progenitor cells in the canine fetal liver

Autor(es)

Tavares, M. R. ^[1] ; de Castro, R. V. G. ^{[2, 1]} ; Pieri, N. C. G. ^[2] ; Cruz, N. R. N. ^[3] ; Martins, D. S. ^{[2, 4]} ; Ambrosio, C. E. ^{[2, 4]} ; Garcia, J. M. ^[1] ; Camplesi, A. C. ^[3] ; Bressan, F. F. ^{[2, 4]} ; Toniollo, G. H. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Sao Paulo, Fac Agr & Anim Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, SP - Brazil

^[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil

^[3] State Univ Sao Paulo, Fac Agr & Anim Sci, Dept Vet Clin & Surg, Jaboticabal, SP - Brazil

^[4] Univ Sao Paulo, Fac Vet Med & Anim Sci, Postgrad Program Anat Domest & Wild Anim, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 133, p. 239-245, DEC 2020.
• Citações Web of Science: 0

Resumo

The liver plays essential roles in human and animal organisms, such as the storage, release, metabolism, and elimination of various endogenous or exogenous substances. Although its vital importance, few treatments are yet available when a hepatic failure occurs, and hence, the use of stem cells has arisen as a possible solution for both human and veterinary medicines. Previous studies have shown the existence of hepatic progenitor cells in human fetuses that were positive for EpCAM and NCAM. There is limited evidence, however, further identification and characterization of these cells in other species. Considering the similarity between dogs and humans regarding physiology, and also the increasing importance of developing new treatments for both veterinary and translational medicine, this study attempted to identify hepatic progenitor cells in canine fetal liver. For that, livers from canine fetuses were collected, cells were isolated by enzymatic digestion and cultured. Cells were characterized regarding morphology and expression of EpCAM, NCAM, Nestin, and Thy-1/CD90 markers. Our results suggest that it is possible to identify hepatic progenitor cells in the canine fetal liver; however, for therapeutic use, further techniques for cellular isolation and culture are necessary to obtain enriched populations of hepatic progenitors from the canine fetal liver. (AU)

• Processo FAPESP: 17/21266-0 - Células progenitoras amnióticas e seu potencial terapêutico
• Beneficiário: Carlos Eduardo Ambrósio
• Modalidade de apoio: Auxílio à Pesquisa - Regular