Periplasm-enriched fractions from Xanthomonas citri subsp. citri type A and X. fuscans subsp. aurantifolii type B present distinct proteomic profiles under in vitro pathogenicity induction

Autor(es):Mostrar menos -

Zandonadi, Flavia S. ^[1] ; Ferreira, Silvia P. ^{[1, 2]} ; Alexandrino, Andre V. ^[1] ; Carnielli, Carolina M. ^{[1, 3]} ; Artier, Juliana ^{[4, 1]} ; Barcelos, Mariana P. ^{[5, 1]} ; Nicolela, Nicole C. S. ^{[5, 1]} ; Prieto, Evandro L. ^[1] ; Goto, Leandro S. ^{[1, 3]} ; Belasque, Jr., Jose ^[6] ; Marques Novo-Mansur, Maria Teresa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Lab Bioquim & Biol Mol Aplicada, UFSCar, Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Dept Quim Analit, Lab Bioanal & Integracao Omica LaBIOm, Inst Quim, Campinas, SP - Brazil

^[3] CNPEM, LNBio, Lab Nacl Biociencias, Campinas, SP - Brazil

^[4] Univ Colorado, Renewable & Sustainable Energy Inst, Boulder, CO 80309 - USA

^[5] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, USP, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 15, n. 12 DEC 18 2020.
• Citações Web of Science: 0

Resumo

The causative agent of Asiatic citrus canker, the Gram-negative bacterium Xanthomonas citri subsp. citri (XAC), produces more severe symptoms and attacks a larger number of citric hosts than Xanthomonas fuscans subsp. aurantifolii XauB and XauC, the causative agents of cancrosis, a milder form of the disease. Here we report a comparative proteomic analysis of periplasmic-enriched fractions of XAC and XauB in XAM-M, a pathogenicity- inducing culture medium, for identification of differential proteins. Proteins were resolved by two-dimensional electrophoresis combined with liquid chromatography-mass spectrometry. Among the 12 proteins identified from the 4 unique spots from XAC in XAM-M (p<0.05) were phosphoglucomutase (PGM), enolase, xylose isomerase (XI), transglycosylase, NAD(P)H-dependent glycerol 3-phosphate dehydrogenase, succinyl-CoA synthetase <beta> subunit, 6-phosphogluconate dehydrogenase, and conserved hypothetical proteins XAC0901 and XAC0223; most of them were not detected as differential for XAC when both bacteria were grown in NB medium, a pathogenicity non-inducing medium. XauB showed a very different profile from XAC in XAM-M, presenting 29 unique spots containing proteins related to a great diversity of metabolic pathways. Preponderant expression of PGM and XI in XAC was validated by Western Blot analysis in the periplasmic-enriched fractions of both bacteria. This work shows remarkable differences between the periplasmic-enriched proteomes of XAC and XauB, bacteria that cause symptoms with distinct degrees of severity during citrus infection. The results suggest that some proteins identified in XAC can have an important role in XAC pathogenicity. (AU)

• Processo FAPESP: 13/16082-6 - Cancro cítrico - Caracterização proteômica e transcricional da interação de Xanthomonas citri e Xanthomonas fuscans com seus hospedeiros cítricos
• Beneficiário: Sílvia Isabel Palma Ferreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/17470-0 - Análise funcional da xilose isomerase na resposta à xilose por Xanthomonas citri e X. fuscans- tipo B e análise proteômica diferencial desta resposta
• Beneficiário: Evandro Luis Prieto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/04546-0 - Análise proteômica diferencial da fração protéica periplasmática das estirpes A, B e C de Xanthomonas axonopodis que diferem na patogenicidade e espectro de citros hospedeiros
• Beneficiário: Flávia da Silva Zandonadi
• Modalidade de apoio: Bolsas no Brasil - Mestrado