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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Toxoplasma gondii isolated from a Brazilian patient with rare pulmonary toxoplasmosis has a novel genotype and is closely related to Amazonian isolates

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Autor(es):
Pena, Hilda Fatima Jesus [1] ; Ferreira, Marina Neves [1] ; Gennari, Solange Maria [2, 1] ; de Andrade, Jr., Heitor Franco [3] ; Meireles, Luciana Regina [3] ; Galisteo, Jr., Andres Jimenez [3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil
[2] Univ Santo Amaro, R Prof Eneas de Siqueira Neto 340, BR-04829300 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Med Trop, Ave Dr Eneas de Carvalho Aguiar 470, BR-05403000 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Parasitology Research; v. 120, n. 3 JAN 2021.
Citações Web of Science: 0
Resumo

Pulmonary toxoplasmosis is rare in immunocompetent patients. Herein, a Toxoplasma gondii strain isolated in Brazil from an immunocompetent patient who had severe pulmonary involvement was biologically and molecularly characterized for the first time. The TgHumIMTBr1 isolate was bioassayed in mice showing a virulent phenotype. Restriction fragment length polymorphism (RFLP) genotyping using 11 markers {[}SAG1, SAG2 (5 ` 3 ` SAG2 and alt. SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3] revealed a new non-archetypal genotype assigned as \#312. Genotyping using ROP18/ROP5 markers exhibited the virulent combination of alleles 4 and 1. Microsatellite analysis using 15 markers (TUB2, W35, TgM-A, B18, B17, M33, IV.1, X1.1, N60, N82, AA, N61, N83, M48 and M102) revealed an atypical genotype with three unique alleles and a rare combination of alleles 246 (W35) and 203 (TgM-A) that is typical of the Amazon region. Non-archetypal genotypes with unique alleles may function in the occurrence of severe toxoplasmosis in immunocompetent patients in Brazil. Attempts to isolate or molecularly detect T. gondii for further genotyping studies would contribute to the understanding of causes related to the severity of toxoplasmosis in immunocompetent patients. (AU)

Processo FAPESP: 15/26294-6 - Pesquisa de protozoários pertencentes aos filos Apicomplexa e Sarcomastigophora em aves rapinantes de vida livre na Cidade de São Paulo
Beneficiário:Hilda Fátima de Jesus Pena
Modalidade de apoio: Auxílio à Pesquisa - Regular