Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and UropathogenicEscherichia coli(aEPEC/UPEC) Strain

Autor(es)

Valiatti, Tiago B. ^{[1, 2]} ; Santos, Fernanda F. ^{[1, 2]} ; Santos, Ana C. M. ^[1] ; Nascimento, Jullia A. S. ^[1] ; Silva, Rosa M. ^[1] ; Carvalho, Eneas ^[3] ; Sinigaglia, Rita ^[4] ; Gomes, Tania A. T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Med, Lab Alerta, Disciplina Infectol, Sao Paulo - Brazil

^[3] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Ctr Microscopia Eletron, Escola Paulista Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, SEP 29 2020.
• Citações Web of Science: 0

Resumo

Hybrid strains ofEscherichia colicombine virulence traits of diarrheagenic (DEC) and extraintestinal pathogenicE. coli(ExPEC), but it is poorly understood whether these combined features improve the virulence potential of such strains. We have previously identified a uropathogenicE. coli(UPEC) strain (UPEC 252) harboring theeaegene that encodes the adhesin intimin and is located in the locus of enterocyte effacement (LEE) pathogenicity island. The LEE-encoded proteins allow enteropathogenicE. coli(EPEC) and enterohemorrhagicE. coli(EHEC) to form attaching and effacing (A/E) lesions in enterocytes. We sought to characterize UPEC 252 through whole-genome sequencing and phenotypic virulence assays. Genome analysis unveiled that this strain harbors a complete LEE region, with more than 97% of identity comparing to E2348/69 (EPEC) and O157:H7 Sakai (EHEC) prototype strains, which was functional, since UPEC 252 expressed the LEE-encoded proteins EspB and intimin and induced actin accumulation foci in HeLa cells. Phylogenetic analysis performed comparing 1,000 single-copy shared genes clustered UPEC 252 with atypical EPEC strains that belong to the sequence type 10, phylogroup A. Additionally, UPEC 252 was resistant to the bactericidal power of human serum and colonized cells of the urinary (T24 and HEK293-T) and intestinal (Caco-2 and LS174T) tracts. Our findings suggest that UPEC 252 is an atypical EPEC strain that emerges as a hybrid strain (aEPEC/UPEC), which could colonize new niches and potentially cause intestinal and extraintestinal infections. (AU)

• Processo FAPESP: 18/17353-7 - ANÁLISE COMPARATIVA DE CARACTERÍSTICAS DE VIRULÊNCIA DE Escherichia coli DIARREIOGÊNICA EM CEPAS DE E. coli ISOLADAS DE PACIENTES COM DIFERENTES TIPOS DE INFECÇÃO URINÁRIA
• Beneficiário: Tânia Aparecida Tardelli Gomes do Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/21947-7 - Análise do genoma e do potencial de virulência de uma cepa de Escherichia coli uropatogênica (UPEC) portadora de marcadores de E. coli diarreiogênica (DEC)
• Beneficiário: Tiago Barcelos Valiatti
• Modalidade de apoio: Bolsas no Brasil - Mestrado