LPMO AfAA9_B and Cellobiohydrolase AfCel6A from A. fumigatus Boost Enzymatic Saccharification Activity of Cellulase Cocktail

Autor(es)

Bernardi, Aline Vianna ^[1] ; Gerolamo, Luis Eduardo ^[1] ; de Gouvea, Paula Fagundes ^[1] ; Yonamine, Deborah Kimie ^[1] ; Pereira, Lucas Matheus Soares ^[1] ; de Oliveira, Arthur Henrique Cavalcante ^[1] ; Uyemura, Sergio Akira ^[2] ; Dinamarco, Taisa Magnani ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 1 JAN 2021.
• Citações Web of Science: 0

Resumo

Cellulose is the most abundant polysaccharide in lignocellulosic biomass, where it is interlinked with lignin and hemicellulose. Bioethanol can be produced from biomass. Since breaking down biomass is difficult, cellulose-active enzymes secreted by filamentous fungi play an important role in degrading recalcitrant lignocellulosic biomass. We characterized a cellobiohydrolase (AfCel6A) and lytic polysaccharide monooxygenase LPMO (AfAA9\_B) from Aspergillus fumigatus after they were expressed in Pichia pastoris and purified. The biochemical parameters suggested that the enzymes were stable; the optimal temperature was similar to 60 degrees C. Further characterization revealed high turnover numbers (k(cat) of 147.9 s(-1) and 0.64 s(-1), respectively). Surprisingly, when combined, AfCel6A and AfAA9\_B did not act synergistically. AfCel6A and AfAA9\_B association inhibited AfCel6A activity, an outcome that needs to be further investigated. However, AfCel6A or AfAA9\_B addition boosted the enzymatic saccharification activity of a cellulase cocktail and the activity of cellulase Af-EGL7. Enzymatic cocktail supplementation with AfCel6A or AfAA9\_B boosted the yield of fermentable sugars from complex substrates, especially sugarcane exploded bagasse, by up to 95%. The synergism between the cellulase cocktail and AfAA9\_B was enzyme- and substrate-specific, which suggests a specific enzymatic cocktail for each biomass by up to 95%. The synergism between the cellulase cocktail and AfAA9\_B was enzyme- and substrate-specific, which suggests a specific enzymatic cocktail for each biomass. (AU)

• Processo FAPESP: 18/11231-7 - Caracterização funcional da endoglucanase GH7 de Aspergillus fumigatus
• Beneficiário: Deborah Kimie Yonamine
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica