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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

LPMO AfAA9_B and Cellobiohydrolase AfCel6A from A. fumigatus Boost Enzymatic Saccharification Activity of Cellulase Cocktail

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Author(s):
Bernardi, Aline Vianna [1] ; Gerolamo, Luis Eduardo [1] ; de Gouvea, Paula Fagundes [1] ; Yonamine, Deborah Kimie [1] ; Pereira, Lucas Matheus Soares [1] ; de Oliveira, Arthur Henrique Cavalcante [1] ; Uyemura, Sergio Akira [2] ; Dinamarco, Taisa Magnani [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 1 JAN 2021.
Web of Science Citations: 0
Abstract

Cellulose is the most abundant polysaccharide in lignocellulosic biomass, where it is interlinked with lignin and hemicellulose. Bioethanol can be produced from biomass. Since breaking down biomass is difficult, cellulose-active enzymes secreted by filamentous fungi play an important role in degrading recalcitrant lignocellulosic biomass. We characterized a cellobiohydrolase (AfCel6A) and lytic polysaccharide monooxygenase LPMO (AfAA9\_B) from Aspergillus fumigatus after they were expressed in Pichia pastoris and purified. The biochemical parameters suggested that the enzymes were stable; the optimal temperature was similar to 60 degrees C. Further characterization revealed high turnover numbers (k(cat) of 147.9 s(-1) and 0.64 s(-1), respectively). Surprisingly, when combined, AfCel6A and AfAA9\_B did not act synergistically. AfCel6A and AfAA9\_B association inhibited AfCel6A activity, an outcome that needs to be further investigated. However, AfCel6A or AfAA9\_B addition boosted the enzymatic saccharification activity of a cellulase cocktail and the activity of cellulase Af-EGL7. Enzymatic cocktail supplementation with AfCel6A or AfAA9\_B boosted the yield of fermentable sugars from complex substrates, especially sugarcane exploded bagasse, by up to 95%. The synergism between the cellulase cocktail and AfAA9\_B was enzyme- and substrate-specific, which suggests a specific enzymatic cocktail for each biomass by up to 95%. The synergism between the cellulase cocktail and AfAA9\_B was enzyme- and substrate-specific, which suggests a specific enzymatic cocktail for each biomass. (AU)

FAPESP's process: 18/11231-7 - Functional characterization of GH7 endoglucanase from Aspergillus fumigatus
Grantee:Deborah Kimie Yonamine
Support Opportunities: Scholarships in Brazil - Scientific Initiation