Immobilization of Eversa(R) Transform via CLEA Technology Converts It in a Suitable Biocatalyst for Biolubricant Production Using Waste Cooking Oil

Autor(es)

Guimaraes, Jose Renato ^[1] ; Miranda, Leticia Passos ^[1] ; Fernandez-Lafuente, Roberto ^{[2, 3]} ; Tardioli, Paulo Waldir ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos DEQ UFSCar, Dept Chem Engn, Postgrad Program Chem Engn PPGEQ, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] CSIC, ICP, Dept Biocatalisis, Campus UAM CSIC, Madrid 28049 - Spain

^[3] King Abdulaziz Univ, External Sci Advisory Acad, Ctr Excellence Bionanosci Res, Jeddah 21589 - Saudi Arabia

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 26, n. 1 JAN 2021.
• Citações Web of Science: 2

Resumo

The performance of the previously optimized magnetic cross-linked enzyme aggregate of Eversa (Eversa-mCLEA) in the enzymatic synthesis of biolubricants by transesterification of waste cooking oil (WCO) with different alcohols has been evaluated. Eversa-mCLEA showed good activities using these alcohols, reaching a transesterification activity with isoamyl alcohol around 10-fold higher than with methanol. Yields of isoamyl fatty acid ester synthesis were similar using WCO or refined oil, confirming that this biocatalyst could be utilized to transform this residue into a valuable product. The effects of WCO/isoamyl alcohol molar ratio and enzyme load on the synthesis of biolubricant were also investigated. A maximum yield of around 90 wt.% was reached after 72 h of reaction using an enzyme load of 12 esterification units/g oil and a WCO/alcohol molar ratio of 1:6 in a solvent-free system. At the same conditions, the liquid Eversa yielded a maximum ester yield of only 34%. This study demonstrated the great changes in the enzyme properties that can be derived from a proper immobilization system. Moreover, it also shows the potential of WCO as a feedstock for the production of isoamyl fatty acid esters, which are potential candidates as biolubricants. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático