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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization of Eversa(R) Transform via CLEA Technology Converts It in a Suitable Biocatalyst for Biolubricant Production Using Waste Cooking Oil

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Author(s):
Guimaraes, Jose Renato [1] ; Miranda, Leticia Passos [1] ; Fernandez-Lafuente, Roberto [2, 3] ; Tardioli, Paulo Waldir [1]
Total Authors: 4
Affiliation:
[1] Fed Univ Sao Carlos DEQ UFSCar, Dept Chem Engn, Postgrad Program Chem Engn PPGEQ, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] CSIC, ICP, Dept Biocatalisis, Campus UAM CSIC, Madrid 28049 - Spain
[3] King Abdulaziz Univ, External Sci Advisory Acad, Ctr Excellence Bionanosci Res, Jeddah 21589 - Saudi Arabia
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 26, n. 1 JAN 2021.
Web of Science Citations: 2
Abstract

The performance of the previously optimized magnetic cross-linked enzyme aggregate of Eversa (Eversa-mCLEA) in the enzymatic synthesis of biolubricants by transesterification of waste cooking oil (WCO) with different alcohols has been evaluated. Eversa-mCLEA showed good activities using these alcohols, reaching a transesterification activity with isoamyl alcohol around 10-fold higher than with methanol. Yields of isoamyl fatty acid ester synthesis were similar using WCO or refined oil, confirming that this biocatalyst could be utilized to transform this residue into a valuable product. The effects of WCO/isoamyl alcohol molar ratio and enzyme load on the synthesis of biolubricant were also investigated. A maximum yield of around 90 wt.% was reached after 72 h of reaction using an enzyme load of 12 esterification units/g oil and a WCO/alcohol molar ratio of 1:6 in a solvent-free system. At the same conditions, the liquid Eversa yielded a maximum ester yield of only 34%. This study demonstrated the great changes in the enzyme properties that can be derived from a proper immobilization system. Moreover, it also shows the potential of WCO as a feedstock for the production of isoamyl fatty acid esters, which are potential candidates as biolubricants. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants