Synthesis and characterization of new biocatalysts formed by nanozeolite and laccase enzymes complexes: their applications in oxidation reactions of glycerol to tartronic acid and mesoxalic acid compounds

• Grant number: 16/24303-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2017
• End date: August 31, 2020
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Jose Geraldo Nery
• Grantee: Alex Henrique Miller
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 18/21483-3 - Laccase/TEMPO-mediated glycerol oxidation spectroscopic and electrochemical assessment, BE.EP.DR

Abstract

The increase in the glycerol production by transesterification of fats or vegetable oils enables the studies of new technological applications for its use as raw material. For every 10 kg of biodiesel synthesized, 1 kg of glycerol is produced. The transformation of glycerol in other products with greater added economic values has gained high industrial interest, once it also favors the biodiesel production economy, and concomitantly, provides the chemical industry with more versatile raw materials or building blocks that can be used to make more complex molecules. Etherification, oxidation, catalytic reforming and esterification, are examples of reaction that glycerol may be applied. The goal of this project is to explore the selective glycerol oxidation to tartronic acid and mesoxalic acid, using new biocatalysts. Both tartronic and mesoxalic acids are compounds with several applications in the pharmaceuticals, cosmetics and fine chemicals industries, what makes the development of new catalytic routes that allows to convert low price biomass to these products a relevant technical-scientific challenge. In this work, the biocatalyst for the selective glycerol oxidation will be different laccase enzymes (oxidoreductase family) immobilized onto zeolitic nanomaterials. Although there are studies of immobilization of enzymes in zeolitic supports in micrometric scale for various purposes, to the best of our knowledge, to the present, there is no report about the use of nano sized zeolites as solid support to the laccase enzymes immobilization and its use for the glycerol oxidation to mesoxalic and tartronic acids. The new biocatalysts will associate the advantages of the heterogeneous catalysts with those of the enzymatic catalysts. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE VASCONCELLOS, ADRIANO; MILLER, ALEX HENRIQUE; ARANDA, DONATO A. G.; NERY, JOSE GERALDO. Biocatalysts based on nanozeolite-enzyme complexes: Effects of alkoxysilane surface functionalization and biofuel production using microalgae lipids feedstock. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 165, p. 150-157, MAY 1 2018. (12/24259-0, 11/10092-4, 11/51851-5, 16/24303-0)

MILLER, ALEX HENRIQUE; DE VASCONCELLOS, ADRIANO; FIELDING, ALISTAIR JOHN; NERY, JOSE GERALDO. Nanozeolites as support for laccase immobilization: Application to mediated glycerol oxidation. APPLIED CATALYSIS A-GENERAL, v. 626, SEP 25 2021. (18/21483-3, 16/24303-0)

MILLER, ALEX H.; NGUYEN, HUONG THI-THANH; NERY, JOSE G.; FIELDING, ALISTAIR J.. Electrochemical sensor based on epoxy-functionalized BEA nanozeolite and graphene oxide modified glassy carbon electrode for bisphenol E determination. Journal of Applied Electrochemistry, v. 53, n. 8, p. 11-pg., 2023-03-18. (16/24303-0, 18/21483-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MILLER, Alex Henrique. Nanozeolites as support for laccases immobilization: application of the biocatalysts to the mediated glycerol oxidation reaction. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.