Biocatalysts based on nanozeolite-enzyme complexes: Effects of alkoxysilane surface functionalization and biofuel production using microalgae lipids feedstock

Author(s)

de Vasconcellos, Adriano ^[1] ; Miller, Alex Henrique ^[1] ; Aranda, Donato A. G. ^[2] ; Nery, Jose Geraldo ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Phys Dept, Lab Clean Energy Technol LACET, Campus Sao Jose do Rio Preto, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Sch Chem, Greentec Lab, BR-21941972 Rio De Janeiro, RJ - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 165, p. 150-157, MAY 1 2018.
• Web of Science Citations: 4

Abstract

Nanozeolites with different crystallographic structures (Nano/TS1, Nano/GIS, Nano/LTA, Nano/BEA, Nano/X, and Nano-X/Ni), functionalized with (3-aminopropyl)trimethoxysilane (APTMS) and crosslinked with glutaraldehyde (GA), were studied as solid supports for Thermomyces lanuginosus lipase (TLL) immobilization. Physicochemical characterizations of the surface-functionalized nanozeolites and nanozeolite-enzyme complexes were performed using XRD, SEM, AFM, ATR-FTIR, and zeta potential measurements. The experimental enzymatic activity results indicated that the nanozeolitic supports functionalized with APTMS and GA immobilized larger amounts of enzymes and provided higher enzymatic activities, compared to unfunctionalized supports. Correlations were observed among the nanozeolite surface charges, the enzyme immobilization efficiencies, and the biocatalyst activities. The catalytic performance and reusability of these enzyme-nanozeolite complexes were evaluated in the ethanolysis transesterification of microalgae oil to fatty acid ethyl esters (FAEEs). TLL immobilized on the nanozeolite supports functionalized with APTMS and GA provided the most efficient biocatalysis, with FAEEs yields above 93% and stability during five reaction cycles. Lower FAEEs yields and poorer catalytic stability were found for nanozeolite-enzyme complexes prepared only by physical adsorption. The findings indicated the viability of designing highly efficient biocatalysts for biofuel production by means of chemical modulation of nanozeolite surfaces. The high biocatalyst catalytic efficiency observed in ethanolysis reactions using a lipid feedstock that does not compete with food production is an advantage that should encourage the industrial application of these biocatalysts. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/24303-0 - Synthesis and characterization of new biocatalysts formed by nanozeolite and laccase enzymes complexes: their applications in oxidation reactions of glycerol to tartronic acid and mesoxalic acid compounds
• Grantee: Alex Henrique Miller
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/24259-0 - Peptide and chitosan conjugates with pharmacological potential: synthesis, prospecting of activity in membrane mimetic systems, and evaluation in cells
• Grantee: Marcia Perez dos Santos Cabrera
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 11/10092-4 - Nanozeolites as solid supports for enzyme immobilization: Synthesis, characterization of complex nanozeolite/enzymes and their application as heterogeneous catalysts for biodiesel production via ethylic route
• Grantee: Adriano de Vasconcellos
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/51851-5 - Syntheses and characterization of nanozeolites-enzymes complexes and their use as heterogeneous catalysts in the production of biodiesel by trans esterification of Palm Oil and algae oils (Dunaliella Salina and Haematococcus pluvialis) with ethanol
• Grantee: Jose Geraldo Nery
• Support type: Regular Research Grants