Nanozeolites as support for laccase immobilization: Application to mediated glycerol oxidation

Author(s)

Miller, Alex Henrique ^{[1, 2]} ; de Vasconcellos, Adriano ^[2] ; Fielding, Alistair John ^[3] ; Nery, Jose Geraldo ^[2]

Affiliation

^[1] Univ York, Dept Chem, York YO10 5DD, N Yorkshire - England

^[2] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci IBILCE, Phys Dept, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Liverpool John Moores Univ, Ctr Nat Prod Discovery, Sch Pharm & Biomol Sci, James Parsons Bldg, Byrom St, Liverpool L3 3AF, Merseyside - England

• Document type: Journal article
• Source: APPLIED CATALYSIS A-GENERAL; v. 626, SEP 25 2021.
• Web of Science Citations: 0

Abstract

This study investigated the use of nanozeolites as support for laccases from P. ostreatus (LPO), Aspergillus sp (LAsp) and A. bisporus (LAB) immobilization applied to 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) mediated glycerol oxidation. Selected complexes led to up to 5% glycerol conversion, and interestingly, up to 100% selectivity to glyceraldehyde after 48 h. Free enzymes led to significantly higher yields (up to 82%) but lacked selectivity when tested under the same conditions. These findings suggest that laccases immobilized into nanozeolites are promising catalysts for the selective oxidation of glycerol. With the aim to understand the different behavior of free or immobilized enzymes, electron paramagnetic resonance (EPR) spectroscopy was applied. A significant shift of the T2 parallel copper hyperfine coupling constant was observed. This suggested a perturbation on the catalytic site after immobilization due to pH variation of the enzymatic microenvironments, thus influencing performance of laccase immobilized on nanozeolites. (AU)

• FAPESP's process: 18/21483-3 - Laccase/TEMPO-mediated glycerol oxidation spectroscopic and electrochemical assessment
• Grantee: Alex Henrique Miller
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/24303-0 - Synthesis and characterization of new biocatalysts formed by nanozeolite and laccase enzymes complexes: their applications in oxidation reactions of glycerol to tartronic acid and mesoxalic acid compounds
• Grantee: Alex Henrique Miller
• Support Opportunities: Scholarships in Brazil - Doctorate