Tuning the properties of carboxymethylchitosan-based porous membranes for potential application as wound dressing

Autor(es)

Bukzem, Andrea de Lacerda ^[1] ; dos Santos, Danilo Martins ^[2] ; Leite, Ilaiali Souza ^[3] ; Inada, Natalia Mayumi ^[3] ; Campana-Filho, Sergio Paulo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 166, p. 459-470, JAN 1 2021.
• Citações Web of Science: 0

Resumo

Wound repair is a complex process that calls for strategies to allow a rapid and effective regeneration of injured skin, which has stimulated the research of advanced wound dressings. Herein, highly porous membranes of N,O-carboxymethylchitosan (CMCh), and poly (vinyl alcohol) (PVA) were successfully prepared via a green and facile freeze-drying method of blend solutions containing CMCh/PVA at weight ratio 25/75. Membranes composed only by CMCh were also prepared and genipin was used for crosslinking. Different contents of TiO2 nanoparticles were incorporated to both type of membranes, which were characterized in terms of morphology, porosity (Phi), swelling capacity (S.C.), mechanical properties, susceptibility to lysozyme degradation and in vitro cytotoxicity toward human fibroblast (HDFn) and keralinocytes (HaCaT) cells. Larger apparent pores were observed in the surface of the genipin-crosslinked CMCh membrane, which resulted in higher porosity (Phi approximate to 76%) and swelling capacity (S.C. approximate to 1720%) as compared to CMCh/PVA membrane (Phi approximate to 68%; S.C. approximate to 1660%). The porosity of both types of membranes decreased upon the addition of TiO2 nanoparticles while swelling capacity increased. Due to their high porosity and swelling capacity, adequate mechanical properties, controlled degradability, and cytocompatibility, such carboxymethylchitosan-based membranes are potentially useful as wound dressings. (C) 2020 Elsevier B.V. All tights reserved. (AU)

• Processo FAPESP: 10/02526-1 - Estudos dos efeitos da irradiação de ultrassom de alta intensidade em suspensões aquosas de quitina e quitosana
• Beneficiário: Sérgio Paulo Campana Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/20973-4 - Nanofibras eletrofiadas coaxiais à base de quitosana para a liberação controlada de antibióticos e aplicações no tratamento de lesões periodontais
• Beneficiário: Danilo Martins dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado