Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning

Autor(es)

Correr, Fernando Henrique ^[1] ; Hosaka, Guilherme Kenichi ^[1] ; Barreto, Fernanda Zatti ^[2] ; Valadao, Isabella Barros ^[2] ; Almeida Balsalobre, Thiago Willian ^[2] ; Furtado, Agnelo ^[3] ; Henry, Robert James ^[3] ; Carneiro, Monalisa Sampaio ^[2] ; Alves Margarido, Gabriel Rodrigues ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo Luiz de Queiroz, Coll Agr, Dept Genet, Av Padua Dias 11, BR-13400970 Piracicaba - Brazil

^[2] Univ Fed Sao Carlos, Ctr Agr Sci, Dept Biotechnol Vegetal & Anim Prod, Rodovia Anhanguera, Km 174, BR-13600970 Araras - Brazil

^[3] Univ Queensland, Queensland Alliance Agr & Food Innovat, Brisbane, Qld 4072 - Australia

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 21, n. 1 SEP 29 2020.
• Citações Web of Science: 0

Resumo

BackgroundThe development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability. Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number. To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.ResultsFirst visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-Seq. We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning. We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes. Genotypes within both biomass groups showed substantial variability in genes involved in photosynthesis. However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group. Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP). Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.ConclusionsTogether, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups. Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes. (AU)

• Processo FAPESP: 15/22993-7 - Transcriptômica para identificação de genes de interesse comercial em cana-de-açúcar
• Beneficiário: Gabriel Rodrigues Alves Margarido
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores