A Multi-Omics Characterization of the Natural Product Potential of Tropical Filamentous Marine Cyanobacteria

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Leao, Tiago ^{[1, 2]} ; Wang, Mingxun ^[1] ; Moss, Nathan ^[2] ; da Silva, Ricardo ^{[1, 3]} ; Sanders, Jon ^{[4, 5, 6]} ; Nurk, Sergey ^[7] ; Gurevich, Alexey ^[7] ; Humphrey, Gregory ^{[4, 5]} ; Reher, Raphael ^[2] ; Zhu, Qiyun ^{[4, 5, 8]} ; Belda-Ferre, Pedro ^{[4, 5]} ; Glukhov, Evgenia ^[2] ; Whitner, Syrena ^{[2, 9]} ; Alexander, Kelsey L. ^{[10, 2]} ; Rex, Robert ^[2] ; Pevzner, Pavel ^[11] ; Dorrestein, Pieter C. ^{[1, 5, 2]} ; Knight, Rob ^{[12, 4, 5]} ; Bandeira, Nuno ^{[1, 11]} ; Gerwick, William H. ^{[1, 2]} ; Gerwick, Lena ^[2]

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^[1] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 - USA

^[2] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92037 - USA

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, NPPNS, BR-14040900 Ribeirao Preto - Brazil

^[4] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 - USA

^[5] Univ Calif San Diego, Ctr Microbiome Innovat, La Jolla, CA 92093 - USA

^[6] Cornell Univ, Cornell Inst Host Microbe Interact & Dis, Ithaca, NY 14850 - USA

^[7] St Petersburg State Univ, Ctr Algorithm Biotechnol, St Petersburg 199004 - Russia

^[8] Arizona State Univ, Sch Life Sci, Tempe, AZ 85281 - USA

^[9] Univ Hawaii, Sch Life Sci, Honolulu, HI 96822 - USA

^[10] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 - USA

^[11] Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 - USA

^[12] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 - USA

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 19, n. 1 JAN 2021.
• Citações Web of Science: 0

Resumo

Microbial natural products are important for the understanding of microbial interactions, chemical defense and communication, and have also served as an inspirational source for numerous pharmaceutical drugs. Tropical marine cyanobacteria have been highlighted as a great source of new natural products, however, few reports have appeared wherein a multi-omics approach has been used to study their natural products potential (i.e., reports are often focused on an individual natural product and its biosynthesis). This study focuses on describing the natural product genetic potential as well as the expressed natural product molecules in benthic tropical cyanobacteria. We collected from several sites around the world and sequenced the genomes of 24 tropical filamentous marine cyanobacteria. The informatics program antiSMASH was used to annotate the major classes of gene clusters. BiG-SCAPE phylum-wide analysis revealed the most promising strains for natural product discovery among these cyanobacteria. LCMS/MS-based metabolomics highlighted the most abundant molecules and molecular classes among 10 of these marine cyanobacterial samples. We observed that despite many genes encoding for peptidic natural products, peptides were not as abundant as lipids and lipopeptides in the chemical extracts. Our results highlight a number of highly interesting biosynthetic gene clusters for genome mining among these cyanobacterial samples. (AU)

• Processo FAPESP: 19/05026-4 - Desenvolvimento de uma plataforma computacional modular extensível para análises de experimentos de metabolômica e metagenômica: Inovando com a descoberta de novas atividades enzimáticas e produtos naturais de interesse farmacêutico derivados
• Beneficiário: Ricardo Roberto da Silva
• Modalidade de apoio: Bolsas no Brasil - Programa BIOTA - Jovens Pesquisadores