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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis

Texto completo
Autor(es):
Meneghetti, Maria C. Z. [1] ; Deboni, Paula [1] ; Palomino, Carlos M. V. [1] ; Braga, Luiz P. [1] ; Cavalheiro, Renan P. [1] ; Viana, Gustavo M. [1] ; Yates, Edwin A. [1, 2] ; Nader, Helena B. [1] ; Lima, Marcelo A. [3, 1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Escola Paulista Med, Inst Farmacol & Biol Mol, Dept Bioquim, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Liverpool, Dept Biochem & Syst Biol, ISMIB, Liverpool L69 7ZB, Merseyside - England
[3] Keele Univ, Sch Life Sci, Mol & Struct Biosci, Huxley Bldg, Keele ST5 5BG, Staffs - England
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Carbohydrate Polymers; v. 255, MAR 1 2021.
Citações Web of Science: 0
Resumo

The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis. (AU)

Processo FAPESP: 17/14179-3 - Tráfego vesicular na biossíntese do heparam sulfato
Beneficiário:Ivarne Luis dos Santos Tersariol
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/03964-6 - Glicosaminoglicanos e proteoglicanos: relação estrutura e função
Beneficiário:Helena Bonciani Nader
Linha de fomento: Auxílio à Pesquisa - Temático