| Full text | |
| Author(s): |
Meneghetti, Maria C. Z.
[1]
;
Deboni, Paula
[1]
;
Palomino, Carlos M. V.
[1]
;
Braga, Luiz P.
[1]
;
Cavalheiro, Renan P.
[1]
;
Viana, Gustavo M.
[1]
;
Yates, Edwin A.
[1, 2]
;
Nader, Helena B.
[1]
;
Lima, Marcelo A.
[3, 1]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Inst Farmacol & Biol Mol, Dept Bioquim, Rua Tres Maio 100, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Liverpool, Dept Biochem & Syst Biol, ISMIB, Liverpool L69 7ZB, Merseyside - England
[3] Keele Univ, Sch Life Sci, Mol & Struct Biosci, Huxley Bldg, Keele ST5 5BG, Staffs - England
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Carbohydrate Polymers; v. 255, MAR 1 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis. (AU) | |
| FAPESP's process: | 17/14179-3 - Vesicle trafficking during heparan sulfate biosynthesis |
| Grantee: | Ivarne Luis dos Santos Tersariol |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/03964-6 - Glycosaminoglycans and proteoglycans: interplay between structure and function |
| Grantee: | Helena Bonciani Nader |
| Support Opportunities: | Research Projects - Thematic Grants |