Nebivolol Prevents Up-Regulation of Nox2/NADPH Oxidase and Lipoperoxidation in the Early Stages of Ethanol-Induced Cardiac Toxicity

Autor(es)

do Vale, Gabriel T. ^{[1, 2]} ; da Silva, Carla B. P. ^{[3, 4]} ; Sousa, Arthur H. ^[4] ; Gonzaga, Natalia A. ^{[2, 4]} ; Parente, Juliana M. ^[2] ; Araujo, Katiuscia M. ^[4] ; Castro, Michele M. ^[2] ; Tirapelli, Carlos R. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estado Minas Gerais UEMG, Passos, MG - Brazil

^[2] Univ Sao Paulo, Programa Posgrad Farmacol, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Programa Posgrad Toxicol, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, USP, Escola Enfermagem Ribeirao Preto, Lab Farmacol Cardiovasc, DEPCH, Ave Bandeirantes 3900, BR-14040902 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cardiovascular Toxicology; v. 21, n. 3, p. 224-235, MAR 2021.
• Citações Web of Science: 0

Resumo

Changes in redox state are described in the early stages of ethanol-induced cardiac toxicity. Here, we evaluated whether nebivolol would abrogate ethanol-induced redox imbalance in the heart. Male Wistar rats were treated with a solution of ethanol (20% v/v) for 3 weeks. Treatment with nebivolol (10 mg/kg/day; p.o. gavage) prevented the increase of both superoxide (O-2(center dot-)) and thiobarbituric acid reactive substances (TBARS) in the left ventricle of rats chronically treated with ethanol. Neither ethanol nor nebivolol affected the expression of Nox4, p47(phox), or Rac-1. Nebivolol prevented ethanol-induced increase of Nox2 expression in the left ventricle. Superoxide dismutase (SOD) activity as well as the concentration of reduced glutathione (GSH) was not altered by ethanol or nebivolol. Augmented catalase activity was detected in the left ventricle of both ethanol- and nebivolol-treated rats. Treatment with nebivolol, but not ethanol increased eNOS expression in the left ventricle. No changes in the activity of matrix metalloproteinase (MMP)2 or in the expressions of MMP2, MMP9, and tissue inhibitor metalloproteinase (TIMP)1 were detected after treatment with ethanol or nebivolol. However, ethanol increased the expression of TIMP2, and this response was prevented by nebivolol. Our results provided novel insights into the mechanisms underlying the early stages of the cardiac injury induced by ethanol consumption. We demonstrated that Nox2/NADPH oxidase-derived ROS play a role in ethanol-induced lipoperoxidation and that this response was prevented by nebivolol. In addition, we provided evidence that MMPs are not activated in the early stages of ethanol-induced cardiac toxicity. (AU)

• Processo FAPESP: 13/15824-9 - Participação da NAD(P)H oxidase na disfunção vascular e aumento da pressão arterial induzido pelo consumo de etanol: envolvimento do estresse oxidativo e da sinalização redox vascular
• Beneficiário: Carlos Renato Tirapelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular