Pregnancy decreases O-GlcNAc-modified proteins in systemic arteries of normotensive and spontaneously hypertensive rats

Autor(es)

Troiano, Jessica A. ^{[1, 2]} ; Potje, Simone R. ^[3] ; Graton, Murilo E. ^{[1, 2]} ; Silva, Daniela S. ^[2] ; da Costa, Rafael M. ^[4] ; Tostes, Rita C. ^[5] ; Antoniali, Cristina ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Programa Posgrad Multictr Ciencias Fisiol, SBFis, Aracatuba, SP - Brazil

^[3] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ribeirao Preto, SP - Brazil

^[4] Univ Fed Goias, Special Acad Unit Hlth Sci, Jatai, Go - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 266, FEB 1 2021.
• Citações Web of Science: 0

Resumo

Aim: We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats. Main methods: O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR). Vascular O-GlcNAc-modified proteins, OGlcNAcase (OGA) and O-GlcNAc transferase (OGT) expression, and OGA activity were analyzed. Concentration-response to phenylephrine (PE) curves were constructed for arteries with and without endothelium. Arteries were treated with vehicle or PugNAc (OGA inhibitor, 100 mu mol/L) in the presence of L-NAME (NOS inhibitor, 100 mu mol/L). Key findings: The content of vascular O-GlcNAc-modified proteins was lower, OGT and OGA expression did not change, and OGA activity was higher in arteries of P-Wistar rats and P-SHR compared to arteries of NP-groups. Reactivity to PE increased in arteries of P-Wistar rats treated with PugNAc compared to vehicle. O-GlcNAcylation of eNOS decreased in P-SHR compared to NP-SHR. PugNAc partially inhibited the effects of endothelium removal and L-NAME on reactivity to PE in arteries of P-Wistar rats. However, PugNAc did not alter reactivity to PE in arteries of P-SHR. Our data showed that pregnancy decreased the content of vascular O-GlcNAc-modified proteins. Significance: Increased OGA activity and decreased O-GlcNAc modification of eNOS boosts eNOS activity in arteries of P-Wistar rats. In P-SHR, altered OGA activity may lower the content of O-GlcNAc-modified proteins, but decreased OGT activity seems a potential mechanism to reduce glycosylation. (AU)

• Processo FAPESP: 16/22180-9 - Avaliação de mecanismos de modificação pós-translacionais que aumentam a biodisponibilidade do óxido nítrico em vasos de ratas normotensas e espontaneamente hipertensas SHR)ao final da prenhez
• Beneficiário: Cristina Antoniali Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/09373-0 - Avaliação de mecanismos de modificação pós-translacional da óxido nítrico sintase endotelial (eNOS) associados a biodisponibilidade do óxido nítrico em ratas espontaneamente hipertensas (SHR) ao final da prenhez
• Beneficiário: Jéssica Antonini Troiano
• Modalidade de apoio: Bolsas no Brasil - Doutorado